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<p>Prebiotic manooligosaccharides (MOS) enhance the immune system by selectively fermenting gut microbiota. While generally produced through enzymatic hydrolysis of natural mannans and agro-industrial residues, forest waste remains an untapped source. We report the extraction of mannan from <i>Pinus taeda</i> residues using steam explosion and its subsequent enzymatic conversion into β-MOS. Monosaccharide analysis confirmed the presence of galactoglucomannan, a characteristic hemicellulose of conifer wood. A β-MOS yield of 11.5 mg per gram of clarified lignocellulosic hydrolysate was achieved, consisting of oligosaccharides with a low degree of polymerization (DP) &lt;5. Within this fraction, DP2 species were the most abundant, reaching a concentration of 8.4 mg/g in the LMW-MOS-CH fraction as quantified by gas chromatography (GC). The major disaccharides identified were β-<span>d</span>-Man<i>p</i>-(1→4)-<span>d</span>-Man<i>p</i> (~50%) and β-<span>d</span>-Glc<i>p</i>-(1→4)-<span>d</span>-Man<i>p</i> (~50%). Besides, nuclear magnetic resonance (NMR) spectroscopy of two purified fractions revealed β-MOS with an average DP of 5 or 6, composed of glucose and mannose in a molar ratio of 1:4 or 1:5, respectively. These findings, coupled with the feasibility of converting cellulose-rich solid residue from steam explosion into second-generation bioethanol, and the antioxidant potential of the phenol-rich fraction recovered during the lignocellulosic hydrolysate clarification, underscore an integrated valorization of forest by-products.</p>

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Enzymatic generation of β-mannooligosaccharides from forest industry by-products

  • 期刊论文

  • 发表日期：

    2026年4月20日

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  • Fabián Mancioni-Font,
  • Yamira Quintero,
  • Matías Cagno,
  • Fernando Bonfiglio,
  • Cecilia Porciúncula - González,
  • Paula López-Revenga,
  • Antonia Montilla,
  • Carolina Fontana,
  • Cecilia Giacomini

摘要

Prebiotic manooligosaccharides (MOS) enhance the immune system by selectively fermenting gut microbiota. While generally produced through enzymatic hydrolysis of natural mannans and agro-industrial residues, forest waste remains an untapped source. We report the extraction of mannan from Pinus taeda residues using steam explosion and its subsequent enzymatic conversion into β-MOS. Monosaccharide analysis confirmed the presence of galactoglucomannan, a characteristic hemicellulose of conifer wood. A β-MOS yield of 11.5 mg per gram of clarified lignocellulosic hydrolysate was achieved, consisting of oligosaccharides with a low degree of polymerization (DP) <5. Within this fraction, DP2 species were the most abundant, reaching a concentration of 8.4 mg/g in the LMW-MOS-CH fraction as quantified by gas chromatography (GC). The major disaccharides identified were β-d-Manp-(1→4)-d-Manp (~50%) and β-d-Glcp-(1→4)-d-Manp (~50%). Besides, nuclear magnetic resonance (NMR) spectroscopy of two purified fractions revealed β-MOS with an average DP of 5 or 6, composed of glucose and mannose in a molar ratio of 1:4 or 1:5, respectively. These findings, coupled with the feasibility of converting cellulose-rich solid residue from steam explosion into second-generation bioethanol, and the antioxidant potential of the phenol-rich fraction recovered during the lignocellulosic hydrolysate clarification, underscore an integrated valorization of forest by-products.