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<p>Prodigiosin (PDG) is a bioactive red tripyrrole pigment produced by <i>Serratia marcescens</i> with significant therapeutic and industrial potential. However, the widespread use of prodigiosin is limited by the high cost associated with its large-scale production. Here, we report the development of a hyper-prodigiosin-producing mutant strain of <i>Serratia</i> sp. MCA-3, using sequential random mutagenesis with a two-fold increase in yield. To enhance production efficiency and sustainability, we tested different low-cost seed-based substrates of which 1% (w/v) chia seed supplementation yielded the highest prodigiosin titer of 1900.25 ± 6.71&#xa0;mg/L. To our knowledge, this constitutes the first report demonstrating chia seed as an effective substrate for prodigiosin biosynthesis. Extraction efficiency was assessed using a variety of solvents, out of which acidified methanol showed maximum absorbance at 533&#xa0;nm. The pigment was purified using silica gel column chromatography and characterized as prodigiosin using high-performance liquid chromatography (HPLC) and high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry (HR-ESI-QTOF-MS), which revealed a molecular ion at m/z 324.2056 [M + H] ⁺ with 97% purity. The antibacterial activity assay showed significant inhibition of <i>Bacillus subtilis</i> MTCC 121, <i>Escherichia coli</i> ATCC 25,922, <i>Staphylococcus aureus</i> ATCC 29,213, and MRSA ATCC 15,187 (methicillin-resistant <i>S. aureus</i>), indicating the promising potential of PDG as a therapeutic agent against infections caused by antibiotic-resistant bacteria. By integrating random mutagenesis with a nutrient-based feeding strategy, we establish an economically viable and environmentally sustainable platform for prodigiosin production.</p>

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Enhanced production and purification of prodigiosin from a sequentially mutated strain of Serratia Sp MCA-3 using chia seeds as a substrate

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Anjali Anjali,
  • Vandana Sharma,
  • Deepika Singh,
  • Saurabh Saran

摘要

Prodigiosin (PDG) is a bioactive red tripyrrole pigment produced by Serratia marcescens with significant therapeutic and industrial potential. However, the widespread use of prodigiosin is limited by the high cost associated with its large-scale production. Here, we report the development of a hyper-prodigiosin-producing mutant strain of Serratia sp. MCA-3, using sequential random mutagenesis with a two-fold increase in yield. To enhance production efficiency and sustainability, we tested different low-cost seed-based substrates of which 1% (w/v) chia seed supplementation yielded the highest prodigiosin titer of 1900.25 ± 6.71 mg/L. To our knowledge, this constitutes the first report demonstrating chia seed as an effective substrate for prodigiosin biosynthesis. Extraction efficiency was assessed using a variety of solvents, out of which acidified methanol showed maximum absorbance at 533 nm. The pigment was purified using silica gel column chromatography and characterized as prodigiosin using high-performance liquid chromatography (HPLC) and high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry (HR-ESI-QTOF-MS), which revealed a molecular ion at m/z 324.2056 [M + H] ⁺ with 97% purity. The antibacterial activity assay showed significant inhibition of Bacillus subtilis MTCC 121, Escherichia coli ATCC 25,922, Staphylococcus aureus ATCC 29,213, and MRSA ATCC 15,187 (methicillin-resistant S. aureus), indicating the promising potential of PDG as a therapeutic agent against infections caused by antibiotic-resistant bacteria. By integrating random mutagenesis with a nutrient-based feeding strategy, we establish an economically viable and environmentally sustainable platform for prodigiosin production.