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<p>Sustainable utilization of agro-industrial residues for microbial enzyme production is a promising avenue. This study demonstrated the utilization of an agro-industrial by product, copra meal, in mannanase production. The present report describes optimized production, characterization and immobilization of β-mannanase on green synthesized iron nanoparticles (FeNPs), mannooligosaccharide (MOS) generation and in silico molecular modeling and docking. Production optimization of β-mannanase from <i>Aspergillus tamarii</i> NKRC1229 resulted in 11.2-fold enhancement in yield (790 U/gds). Immobilization of partially purified mannanase (75&#xa0;kDa) onto biogenic FeNPs lead to enhancement in its thermal and pH stability with optimum activity at pH 5.0 and 70&#xa0;°C. The immobilized enzyme retained over 96.6% activity after 80&#xa0;min at 50&#xa0;°C, compared to 87.09% in free form. Furthermore, the immobilized mannanase also displayed tolerance to various inhibitors, including β-mercaptoethanol, Zn²⁺, SDS, and sodium azide and retained over 50% activity after seven cycles. High performance liquid chromatography (HPLC) analysis showed that the β-mannanase generated MOS (M3, M4, M5 and M6) by hydrolyzing Locust bean gum, Konjac gum, and Guar gum yielding 46.32&#xa0;mg/mL, 34.80&#xa0;mg/mL, and 1.37&#xa0;mg/mL MOS, respectively. The structural and functional properties of β-mannanase along with substrate binding preferences were elucidated by in silico study. The structural quality of the model was evaluated using Ramachandran plot and Z-score while, molecular docking results revealed strong interactions between β-mannanase and mannans, with a binding affinity of -7.8 Kcal/mol with galactomannan, complementing experimental data. Effective immobilization of β-mannanase on iron nanoparticles and its recyclability in MOS generation from low-cost mannans holds potential in prebiotic synthesis and waste valorization.</p>

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Mannanase production from Aspergillus tamarii NKRC1229 using copra meal and its immobilization for mannooligosaccharide (MOS) generation

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Dharini Pandey,
  • Naveen Kango

摘要

Sustainable utilization of agro-industrial residues for microbial enzyme production is a promising avenue. This study demonstrated the utilization of an agro-industrial by product, copra meal, in mannanase production. The present report describes optimized production, characterization and immobilization of β-mannanase on green synthesized iron nanoparticles (FeNPs), mannooligosaccharide (MOS) generation and in silico molecular modeling and docking. Production optimization of β-mannanase from Aspergillus tamarii NKRC1229 resulted in 11.2-fold enhancement in yield (790 U/gds). Immobilization of partially purified mannanase (75 kDa) onto biogenic FeNPs lead to enhancement in its thermal and pH stability with optimum activity at pH 5.0 and 70 °C. The immobilized enzyme retained over 96.6% activity after 80 min at 50 °C, compared to 87.09% in free form. Furthermore, the immobilized mannanase also displayed tolerance to various inhibitors, including β-mercaptoethanol, Zn²⁺, SDS, and sodium azide and retained over 50% activity after seven cycles. High performance liquid chromatography (HPLC) analysis showed that the β-mannanase generated MOS (M3, M4, M5 and M6) by hydrolyzing Locust bean gum, Konjac gum, and Guar gum yielding 46.32 mg/mL, 34.80 mg/mL, and 1.37 mg/mL MOS, respectively. The structural and functional properties of β-mannanase along with substrate binding preferences were elucidated by in silico study. The structural quality of the model was evaluated using Ramachandran plot and Z-score while, molecular docking results revealed strong interactions between β-mannanase and mannans, with a binding affinity of -7.8 Kcal/mol with galactomannan, complementing experimental data. Effective immobilization of β-mannanase on iron nanoparticles and its recyclability in MOS generation from low-cost mannans holds potential in prebiotic synthesis and waste valorization.