Scholarlink

<p>Environmental pollution resulting from the indiscriminate discharge of toxic chromium (Cr) metal at elevated concentrations from tanneries has been a growing concern. High-throughput amplicon sequencing of the 16&#xa0;S rRNA gene (V3-V4 region) was performed on an Illumina MiSeq sequencing platform to gain insights into the bacterial communities residing in Cr-contaminated tannery waste dump sites and genes related to heavy metal resistance. A diverse range of 16&#xa0;S rRNA genes identified 243,011 operational taxonomic units (OTUs), indicating 27 bacterial phyla, 106 orders, 197 families, and 343 genera. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were identified as the major phyla in all the three SG1 (high Cr-contaminated site-1220.63&#xa0;mg/kg), TSS2 (moderate Cr contaminated site-790.54&#xa0;mg/kg), and TSS3 (low Cr contaminated site-315.32&#xa0;mg/kg) samples, accounting for over 75% of the total bacterial communities. Core microbiome analysis revealed that <i>Pseudomonas</i>,<i> Flavobacterium</i>,<i> Sphingobacterium</i>,<i> Bacillus</i>,<i> Prevotella</i>,<i> Psychrobacter</i>,<i> Paenibacillus</i>,<i> Lysinibacillus</i>, and <i>Brevundimonas</i> were the abundant genera. Moreover, Clusters of Orthologous Groups (COG) analysis manifested 956 predicted functions out of which 14.13 and 8.18% belonged to amino acid metabolism and coenzyme transport respectively, in the SG1. While 17.55 and 17.23% were involved in energy production and conversion, and amino acid transport and metabolism respectively, in the TSS2. And, 11.57 and 10.52% are dominantly tangled in amino acid metabolism, and ribosomal structure respectively, in the TSS3. Kyoto Encyclopaedia of Genes and Genomes (KEGG) functional category exhibited 6885 functions. Out of which, 49.45–51.73% were involved in metabolism, 7.19–9.95% in environmental and information processing, and 10.94–19.94% belonged to a genetic information processing nature. Further, functional analysis using the BacMet2.0 database revealed the relative abundance of genes associated with heavy metal resistance; such as <i>copA</i>,<i> copB</i>,<i> copC</i>,<i> copS</i>,<i> dsbA</i>,<i> arsR</i>, and <i>chrA</i>. The study provides a comprehensive view of the structural and functional dynamics of the bacterial communities residing in different levels of Cr-contaminated tannery dumpsites, which may help in devising bacterial remediation strategies for these sites.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Soil bacterial diversity and its functions shift under varying chromium concentration in tannery waste dumpsite

  • 期刊论文

  • 发表日期：

    2025年9月23日

查看全文
  • Sandhya Mishra,
  • Priyamvada Mishra,
  • Priya Dubey,
  • Mariya Naseem,
  • Anju Patel,
  • Meher H. Asif,
  • Pankaj Kumar Srivastava

摘要

Environmental pollution resulting from the indiscriminate discharge of toxic chromium (Cr) metal at elevated concentrations from tanneries has been a growing concern. High-throughput amplicon sequencing of the 16 S rRNA gene (V3-V4 region) was performed on an Illumina MiSeq sequencing platform to gain insights into the bacterial communities residing in Cr-contaminated tannery waste dump sites and genes related to heavy metal resistance. A diverse range of 16 S rRNA genes identified 243,011 operational taxonomic units (OTUs), indicating 27 bacterial phyla, 106 orders, 197 families, and 343 genera. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were identified as the major phyla in all the three SG1 (high Cr-contaminated site-1220.63 mg/kg), TSS2 (moderate Cr contaminated site-790.54 mg/kg), and TSS3 (low Cr contaminated site-315.32 mg/kg) samples, accounting for over 75% of the total bacterial communities. Core microbiome analysis revealed that Pseudomonas, Flavobacterium, Sphingobacterium, Bacillus, Prevotella, Psychrobacter, Paenibacillus, Lysinibacillus, and Brevundimonas were the abundant genera. Moreover, Clusters of Orthologous Groups (COG) analysis manifested 956 predicted functions out of which 14.13 and 8.18% belonged to amino acid metabolism and coenzyme transport respectively, in the SG1. While 17.55 and 17.23% were involved in energy production and conversion, and amino acid transport and metabolism respectively, in the TSS2. And, 11.57 and 10.52% are dominantly tangled in amino acid metabolism, and ribosomal structure respectively, in the TSS3. Kyoto Encyclopaedia of Genes and Genomes (KEGG) functional category exhibited 6885 functions. Out of which, 49.45–51.73% were involved in metabolism, 7.19–9.95% in environmental and information processing, and 10.94–19.94% belonged to a genetic information processing nature. Further, functional analysis using the BacMet2.0 database revealed the relative abundance of genes associated with heavy metal resistance; such as copA, copB, copC, copS, dsbA, arsR, and chrA. The study provides a comprehensive view of the structural and functional dynamics of the bacterial communities residing in different levels of Cr-contaminated tannery dumpsites, which may help in devising bacterial remediation strategies for these sites.

Graphical Abstract