<p>Banana is an important commercial fruit crop grown in the tropical and subtropical regions of the world. India is the world’s largest producer and exporter of banana, accounting for 25.56% of the global production. Various reports from several parts of India suggest that banana orchards are heavily infested with plant-parasitic nematodes, including Root-knot nematodes (RKN), Burrowing nematodes, Lesion nematodes and Spiral nematodes. However, limited information is available on such infestation in Maharashtra, particularly in Jalgaon area. So, a comprehensive study across 20 orchards aimed to assess nematode infestationsin three important banana-producing blocks of Jalgaon, involving interviews with farmers growers were undertaken. All the samples were found positive for all the above-mentioned nematodes, with <i>Helicotylenchus</i> being the most abundant genera. The infected young plants did not show any disease symptoms, but the older plants showed yellowing and browning of the leaves with necrosis in the margins, stunted plant growth, drooping of leaves, and roots with simple and compound galls depending on the severity of the infection. Morphological identification by observing the RKN female’s perineal patterns and juveniles of other nematodes confirmed their identity. Our study confirms the presence of nematode parasites of banana in previously unreported areas of Maharashtra. More information on the distribution of such nematodes might be useful to prevent their further spread to newer areas. Though there is a strong linkage between farmers and agricultural establishments, farmers have limited awareness of nematode infestations. More comprehensive studies and improved communication are crucial for implementing effective nematode mitigation strategies and safeguarding banana yield.</p>