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<p>Ascochyta blight, caused by <i>Ascochyta rabiei</i>, remains a major constraint to chickpea cultivation in temperate regions. To gain deeper insight into host–pathogen interactions, a comparative proteomic analysis (2D-MALDI-TOF/PMF) was performed to assess infection-induced changes in protein expression. Two chickpea cultivars-ICC-4991 (susceptible) and PBG-5 (resistant) were examined at 24, 48, and 72&#xa0;h post-inoculation. Although both cultivars showed similar protein profiles prior to infection, pronounced differences appeared afterward, especially within the pH 4–7 range. Differentially expressed proteins were linked to pathogenesis (45.83%), defence responses (20.83%), ROS scavenging (12.50%), signalling functions (12.50%), and antimicrobial or structural activity (4.17%). The resistant cultivar PBG-5 exhibited strong upregulation of several key proteins, notably Vicilin-like antimicrobial peptides (13.48-fold), Glucan endo-1,3-β-glucosidase (10.48-fold), and Peroxidase 34 (5.78-fold), while down regulated proteins were fewer and less significant. These results deepen the understanding of chickpea responses to <i>A. rabiei</i> and highlight potential molecular targets for breeding resistant cultivars.</p>

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Comparative host-proteome profiling of chickpea resistance and susceptibility to Ascochyta rabiei

  • 期刊论文

  • 发表日期：

    2026年7月15日

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  • Lakshmaiah Manjunatha,
  • Vaibhav Kumar,
  • Yogesh Kumar,
  • A. K. Srivastava,
  • G. S. Madhu,
  • V. Venkataravanappa

摘要

Ascochyta blight, caused by Ascochyta rabiei, remains a major constraint to chickpea cultivation in temperate regions. To gain deeper insight into host–pathogen interactions, a comparative proteomic analysis (2D-MALDI-TOF/PMF) was performed to assess infection-induced changes in protein expression. Two chickpea cultivars-ICC-4991 (susceptible) and PBG-5 (resistant) were examined at 24, 48, and 72 h post-inoculation. Although both cultivars showed similar protein profiles prior to infection, pronounced differences appeared afterward, especially within the pH 4–7 range. Differentially expressed proteins were linked to pathogenesis (45.83%), defence responses (20.83%), ROS scavenging (12.50%), signalling functions (12.50%), and antimicrobial or structural activity (4.17%). The resistant cultivar PBG-5 exhibited strong upregulation of several key proteins, notably Vicilin-like antimicrobial peptides (13.48-fold), Glucan endo-1,3-β-glucosidase (10.48-fold), and Peroxidase 34 (5.78-fold), while down regulated proteins were fewer and less significant. These results deepen the understanding of chickpea responses to A. rabiei and highlight potential molecular targets for breeding resistant cultivars.