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<p>Planting material plays an important role in the production of fruit crops. Inadequate availability of quality planting material is one of the important deterring factors in development of a sound fruit industry. Keeping this in mind we produce a citrus graft-transmissible pathogens CGTPs free planting material for the north east India, where the citrus production is reducing due to decline cause by CGTPs. Khasi mandarin (KM) (<i>Citrus reticulata</i>) is a vital fruit crop in Northeast India, contributing significantly to regional livelihoods. However, its production is severely threatened by citrus graft-transmissible pathogens (CGTPs), including Citrus tristeza virus (CTV), Indian citrus ringspot virus (ICRSV), Citrus yellow mosaic virus (CYMV), and Huanglongbing (HLB) bacteria. This study conducted a comprehensive survey across Assam, Manipur, and Meghalaya to assess CGTP incidence using ELISA (CTV) and PCR-based detection. Among the 77 KM samples analyzed, 67.5% were infected with CTV, 5.2% with ICRSV, and 53.2% with HLB, while no CYMV infections were detected. Regional infection rates ranged from 63.2% to 75.0% for CTV, 50.0% to 57.8% for HLB, and 5.0% to 5.26% for ICRSV. Nine samples were free of all four CGTPs. The complete coat protein (CP) gene of CTV (672nt), partial 16&#xa0;S rRNA of HLB (451 nt), and partial CP of ICRSV (530 nt) were amplified, cloned, and sequenced, confirming their phylogenetic placement among known isolates. Disease-free KM samples were grafted onto an indicator host, and after six months, no symptoms emerged, validating their CGTP-free status. These pathogen-free mother stocks were utilized for scion propagation via grafting onto rough lemon rootstock as a propagation rootstock, ensuring the production of clean planting materials. This study highlights the urgent need for CGTP surveillance and disease-free propagation strategies to sustain KM production in Northeast India.</p>

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Identification of virus and greening bacteria-free Khasi mandarin mother stock and development of strategy for the production of planting materials for Northeast India

  • 期刊论文

  • 发表日期：

    2026年7月18日

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  • Halima Khatoon,
  • Shaivya Singh,
  • Dharmappa D. Chavan,
  • Marimuthu Elangovan,
  • Bharat Raj Meena,
  • Lalit Patil,
  • Susheel Kumar Sharma,
  • Anirban Roy,
  • Sanjay Kumar Singh,
  • Shilpi Agarwal,
  • Nirmal Mazumder,
  • Anup Kumar Bhattacherjee,
  • Kajal Kumar Biswas

摘要

Planting material plays an important role in the production of fruit crops. Inadequate availability of quality planting material is one of the important deterring factors in development of a sound fruit industry. Keeping this in mind we produce a citrus graft-transmissible pathogens CGTPs free planting material for the north east India, where the citrus production is reducing due to decline cause by CGTPs. Khasi mandarin (KM) (Citrus reticulata) is a vital fruit crop in Northeast India, contributing significantly to regional livelihoods. However, its production is severely threatened by citrus graft-transmissible pathogens (CGTPs), including Citrus tristeza virus (CTV), Indian citrus ringspot virus (ICRSV), Citrus yellow mosaic virus (CYMV), and Huanglongbing (HLB) bacteria. This study conducted a comprehensive survey across Assam, Manipur, and Meghalaya to assess CGTP incidence using ELISA (CTV) and PCR-based detection. Among the 77 KM samples analyzed, 67.5% were infected with CTV, 5.2% with ICRSV, and 53.2% with HLB, while no CYMV infections were detected. Regional infection rates ranged from 63.2% to 75.0% for CTV, 50.0% to 57.8% for HLB, and 5.0% to 5.26% for ICRSV. Nine samples were free of all four CGTPs. The complete coat protein (CP) gene of CTV (672nt), partial 16 S rRNA of HLB (451 nt), and partial CP of ICRSV (530 nt) were amplified, cloned, and sequenced, confirming their phylogenetic placement among known isolates. Disease-free KM samples were grafted onto an indicator host, and after six months, no symptoms emerged, validating their CGTP-free status. These pathogen-free mother stocks were utilized for scion propagation via grafting onto rough lemon rootstock as a propagation rootstock, ensuring the production of clean planting materials. This study highlights the urgent need for CGTP surveillance and disease-free propagation strategies to sustain KM production in Northeast India.