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<p>Tomato (<i>Solanum lycopersicum</i> L.) is a widely cultivated crop in India, but its productivity is increasingly threatened by viral diseases. During sample collection from a tomato field in Thondamuthur village, Coimbatore district, Tamil Nadu, tomato plants exhibiting yellowing, interveinal chlorosis, mottling, and leaf curling were observed. Molecular assays using RT-PCR with universal <i>Crinivirus</i> and tomato chlorosis virus (ToCV)-specific primers revealed the presence of ToCV in symptomatic samples. Further confirmation was achieved through Sanger sequencing, BLAST analysis, and phylogenetic reconstruction of the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) gene regions, which showed high similarity to East Asian ToCV isolates. Serological detection using DAS-ELISA also supported the molecular findings. This is the first confirmed report of ToCV infecting tomato in Southern India. The results highlight the growing significance of criniviruses in solanaceous crops and emphasize the need for rapid diagnostic tools, whitefly vector identification, and integrated management strategies to constrain the spread of ToCV.</p>

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Emergence of tomato chlorosis virus in southern India: a new threat to tomato cultivation

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • B. S. Bharath,
  • Gandhi Karthikeyan,
  • Nagendran Krishnan,
  • N. Boopathi,
  • K. Sivagnanapazham

摘要

Tomato (Solanum lycopersicum L.) is a widely cultivated crop in India, but its productivity is increasingly threatened by viral diseases. During sample collection from a tomato field in Thondamuthur village, Coimbatore district, Tamil Nadu, tomato plants exhibiting yellowing, interveinal chlorosis, mottling, and leaf curling were observed. Molecular assays using RT-PCR with universal Crinivirus and tomato chlorosis virus (ToCV)-specific primers revealed the presence of ToCV in symptomatic samples. Further confirmation was achieved through Sanger sequencing, BLAST analysis, and phylogenetic reconstruction of the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) gene regions, which showed high similarity to East Asian ToCV isolates. Serological detection using DAS-ELISA also supported the molecular findings. This is the first confirmed report of ToCV infecting tomato in Southern India. The results highlight the growing significance of criniviruses in solanaceous crops and emphasize the need for rapid diagnostic tools, whitefly vector identification, and integrated management strategies to constrain the spread of ToCV.