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<p>This study assessed the shelf life and viability of talc-based bioformulations containing <i>Trichoderma</i> spp. and <i>Bacillus</i> spp. under different storage conditions. Initial spore counts ranged from 9.1 × 10¹¹ to 9.3 × 10¹⁰ for <i>Trichoderma</i> spp. and 9.3 × 10⁹ to 8.2 × 10¹⁰ for <i>Bacillus</i> spp. Formulations were stored in polybags, paper, and cloth bags at temperatures ranging from 5&#xa0;°C to 45&#xa0;°C, with spore viability monitored over one year. <i>Trichoderma</i> isolates remained viable for up to 300 days at ≤25&#xa0;°C and 330 days at 0&#xa0;°C, with final CFU/g counts of T3 (2.8 × 10⁶), T12 (4.4 × 10⁶), T15 (7.1 × 10⁶), and T19 (2.7 × 10⁶). Similarly, <i>Bacillus</i> isolates remained viable for up to 300 days at 0&#xa0;°C, with final counts of B9 (2.1 × 10⁶), B23 (1.8 × 10⁶), B15 (4.4 × 10⁶), and B17 (3.6 × 10⁶). Packaging material influenced shelf life with polybags retaining higher spore counts than paper and cloth bags. These findings offer key insights into the viability of bioformulations, reinforcing their potential in sustainable biological control strategies.</p>

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Effect of packing material and storage temperature on the viability of talc-based bioformulations of Trichoderma spp. and Bacillus spp

  • 期刊论文

  • 发表日期：

    2026年3月21日

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  • Sukhman Kaur Aulakh,
  • Daljeet Singh Buttar,
  • Ajay Kumar Choudhary

摘要

This study assessed the shelf life and viability of talc-based bioformulations containing Trichoderma spp. and Bacillus spp. under different storage conditions. Initial spore counts ranged from 9.1 × 10¹¹ to 9.3 × 10¹⁰ for Trichoderma spp. and 9.3 × 10⁹ to 8.2 × 10¹⁰ for Bacillus spp. Formulations were stored in polybags, paper, and cloth bags at temperatures ranging from 5 °C to 45 °C, with spore viability monitored over one year. Trichoderma isolates remained viable for up to 300 days at ≤25 °C and 330 days at 0 °C, with final CFU/g counts of T3 (2.8 × 10⁶), T12 (4.4 × 10⁶), T15 (7.1 × 10⁶), and T19 (2.7 × 10⁶). Similarly, Bacillus isolates remained viable for up to 300 days at 0 °C, with final counts of B9 (2.1 × 10⁶), B23 (1.8 × 10⁶), B15 (4.4 × 10⁶), and B17 (3.6 × 10⁶). Packaging material influenced shelf life with polybags retaining higher spore counts than paper and cloth bags. These findings offer key insights into the viability of bioformulations, reinforcing their potential in sustainable biological control strategies.