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<p>Rice is the most important food crop in the world, whose production is affected by several biotic stresses. Sheath blight of rice caused by <i>Rhizoctonia solani</i> Kühn is the second most significant fungal threat to the crop. The lack of absolute resistance makes its management challenging. Rice exhibits a large number of defense actions and produces pathogen-related proteins. The present study was conducted with the objective to characterize the biochemical response of rice genotypes to the infection by <i>R. solani</i>. After two years of screening for sheath blight response using a standard assessment technique, a few recombinant inbred lines along with resistant and susceptible checks were screened for their phenotypic response for computing the area under the disease progress curve and the percentage of disease index. Plant samples were taken at 1 DAI, 2 DAI, 4 DAI and 7 DAI for evaluation of their biochemical activities of chitinase, catalase, phenyl ammonia lyase (PAL), and β-1,3-Glucanase, which showed that the degree of expressiveness continues to rise with progression after inoculation. The activity of these enzymes was significantly higher in resistant lines and resistant checks (Tetep and Pusa 1908-13-12-5) as compared to the susceptible RILs and checks (PB 1 and PB 1121). This study clearly demonstrates that the changes in enzymatic activity of three key enzymes namely, chitinase, catalase, and β-1,3-glucanase involved in modulation of resistance to sheath blight which will be helpful in differentiating resistant and susceptible rice genotypes.</p>

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Biochemical characterization of rice recombinant inbred lines for resistance against Rhizoctonia solani

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • Manoranjan Senapati,
  • Ajit Tiwari,
  • Bishnu Maya Bashyal,
  • Ranjith Kumar Ellur,
  • Prolay Kumar Bhowmick,
  • Haritha Bollinedi,
  • K. K. Vinod,
  • Ashok Kumar Singh,
  • S. Gopala Krishnan

摘要

Rice is the most important food crop in the world, whose production is affected by several biotic stresses. Sheath blight of rice caused by Rhizoctonia solani Kühn is the second most significant fungal threat to the crop. The lack of absolute resistance makes its management challenging. Rice exhibits a large number of defense actions and produces pathogen-related proteins. The present study was conducted with the objective to characterize the biochemical response of rice genotypes to the infection by R. solani. After two years of screening for sheath blight response using a standard assessment technique, a few recombinant inbred lines along with resistant and susceptible checks were screened for their phenotypic response for computing the area under the disease progress curve and the percentage of disease index. Plant samples were taken at 1 DAI, 2 DAI, 4 DAI and 7 DAI for evaluation of their biochemical activities of chitinase, catalase, phenyl ammonia lyase (PAL), and β-1,3-Glucanase, which showed that the degree of expressiveness continues to rise with progression after inoculation. The activity of these enzymes was significantly higher in resistant lines and resistant checks (Tetep and Pusa 1908-13-12-5) as compared to the susceptible RILs and checks (PB 1 and PB 1121). This study clearly demonstrates that the changes in enzymatic activity of three key enzymes namely, chitinase, catalase, and β-1,3-glucanase involved in modulation of resistance to sheath blight which will be helpful in differentiating resistant and susceptible rice genotypes.