<p>White pattypan squash (<i>Cucurbita pepo</i>) plants exhibiting symptoms of yellow mosaic disease were collected from the experimental plot at the Central Horticultural Experiment Station (CHES) in the Kodagu district of Karnataka, India. To identify the causal pathogen, polymerase chain reaction (PCR) was performed using begomovirus-specific primers targeting the DNA-A and DNA-B genomic components. The amplified PCR products were subsequently cloned and sequenced. Complete genome sequence analysis revealed that the white pattypan squash isolate (WPS1) shared a high nucleotide sequence identity of 98% with the DNA-A component and 96% with the DNA-B component of tomato leaf curl New Delhi virus (ToLCNDV, <i>Begomovirus solanumdelhiense</i>) isolates infecting different crops in Indian subcontinents. Recombination breakpoint analysis further indicated that the genome of ToLCNDV infecting white pattypan squash is recombinant, involving already known ToLCNDV isolates and tomato leaf curl Palampur virus (ToLCPalV, <i>Begomovirus solanumpalampurense</i>), both of which are known to infect cucurbits. This study represents the first report of ToLCNDV infecting white pattypan squash in India, highlighting the expanding host range of this virus. These findings emphasize the need for continuous surveillance and management strategies to mitigate the spread of ToLCNDV in cucurbit crops.</p>