In vitro and in-vivo efficacy of Cymbopogon citratus (DC) Stapf (Lemongrass) extract against Colletotrichum gloeosporioides Penz for post-harvest management of mango anthracnose
摘要
The study sought to evaluate the fungicidal properties of Cymbopogon citratus extracts (CCE) against Colletotrichum gloeosporioides through both in vitro and in vivo investigations. In the in-vitro assay, CCE, at concentrations between 1.75 and 2.50 mL/L, utterly prevent the growth and development of C. gloeosporioides. Notably, treatment with CCE induced mycelial blast, conidial swelling, and cytoplasmic discharge in C. gloeosporioides. The minimum inhibitory concentration (MIC) of CCE was determined to be 1.75 mL/L. Furthermore, CCE was identified as a potentially curative, preventative, and simultaneous application for mango fruit. Comparative analysis with the synthetic fungicide (Mancozeb) indicated that CCE exhibited superior efficacy and potency. This enhanced effectiveness may be attributed to the synergistic interactions of the major and minor compounds of CCE, resulting in amplified toxic effects and subsequent fungal death. The GCMS analysis of CCE revealed twenty-six compounds, with 2-Monopalmitin (17.62%), α-Monostearin (10.39%), Geranic acid (9.91%), α-Citral (9.39%), β-Citral (7.25%), and Palmitic acid (6.21%) identified as the major compound.