First record of tomato leaf curl Kerala virus and betasatellite associated with yellow vein mosaic disease of beet root (Beta vulgaris L. ssp. vulgaris) in India

Beet root plant exhibiting different kinds of symptoms were collected from two locations of Karnataka State (India). The presence of causal agent in the infected samples was assayed by PCR using begomovirus specific primers. The partial genome amplification from the ten infected beet root samples was cloned and sequenced. The Sequence Demarcation Tool (SDT) analysis revealed that infected beet root samples were closely related (> 95% nucleotide identity) to tomato leaf curl Kerala virus (ToLCKeV) reported from Pakistan. A comparison of complete genomes (DNA-A) of the five representatives ((BR1, BR2, BR3, BR4 and BR5) isolates infecting beet root revealed that more than 97% nt identity with ToLCKeV. Further full-length genome sequence of DNA satellite shared highest nucleotide (nt) identity of 98.5% with papaya leaf curl betasatellite (PaLCuB). This is the first record of ToLCKeV and PaLCuB associated with yellow vein mosaic disease of beet root plant from India.