Scholarlink

<p>Halophytes are a promising food source thanks to their excellent nutritional value and tolerance of salt. <i>Atriplex</i> species are widely used in traditional cuisine and medicine. These plants are rich in protein, fibre, vitamins, minerals, trace elements and phytochemicals, and are emerging as a promising dietary alternative that supports overall health and immunity. This study investigated the phytochemical content of the hydroethanolic extract of <i>Atriplex nitens</i> Schkuhr (ANS) for the first time using LC-ESI/MS/MS. The study also examined it’s in vitro enzyme inhibition (xanthine oxidase (XO), α-glucosidase and α-amylase), as well as its antibacterial, antibiofilm, anti-inflammatory and cytotoxic activities (against HT29 colorectal cancer cells and L929 mouse fibroblast cells) for the first time. LC-ESI/MS/MS analysis revealed that gentisic acid and salicylic acid were the predominant compounds. ANS extract demonstrated stronger inhibitory activity against α-glucosidase (2.01 ± 0.22&#xa0;µg/mL), α-amylase (11.07 ± 0.90&#xa0;µg/mL), and xanthine oxidase (6.69 ± 0.12&#xa0;µg/mL) enzymes under tested in vitro conditions compared to reference drugs. In addition, it showed higher antibacterial activity against <i>K. pneumoniae</i> than other bacterial strains, while its anti-biofilm activity was high against <i>S. aureus</i>. This indicated that ANS exhibited cytotoxic effects on HT29 cells, while it may have less effect on L929. Additionally, molecular docking was used to investigate the in vitro interactions of major compounds with enzymes. The results of binding affinity tests showed that gentisic/salicylic acid adducts had a higher binding affinity than either compound alone.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Investigation of the In Vitro Antibacterial, Antibiofilm, Anti-Inflammatory, Enzyme Inhibition and Cytotoxic Activities of the Hydroethanolic Extract of Atriplex nitens Schkuhr

  • 期刊论文

  • 发表日期：

    2026年5月23日

查看全文
  • Semiha Yenigun,
  • Yunus Başar,
  • Ayse Karacalı Tunc,
  • Aslı Yıldırım Kocaman,
  • Süleyman Temel,
  • Ibrahim Demirtas,
  • Tevfik Ozen

摘要

Halophytes are a promising food source thanks to their excellent nutritional value and tolerance of salt. Atriplex species are widely used in traditional cuisine and medicine. These plants are rich in protein, fibre, vitamins, minerals, trace elements and phytochemicals, and are emerging as a promising dietary alternative that supports overall health and immunity. This study investigated the phytochemical content of the hydroethanolic extract of Atriplex nitens Schkuhr (ANS) for the first time using LC-ESI/MS/MS. The study also examined it’s in vitro enzyme inhibition (xanthine oxidase (XO), α-glucosidase and α-amylase), as well as its antibacterial, antibiofilm, anti-inflammatory and cytotoxic activities (against HT29 colorectal cancer cells and L929 mouse fibroblast cells) for the first time. LC-ESI/MS/MS analysis revealed that gentisic acid and salicylic acid were the predominant compounds. ANS extract demonstrated stronger inhibitory activity against α-glucosidase (2.01 ± 0.22 µg/mL), α-amylase (11.07 ± 0.90 µg/mL), and xanthine oxidase (6.69 ± 0.12 µg/mL) enzymes under tested in vitro conditions compared to reference drugs. In addition, it showed higher antibacterial activity against K. pneumoniae than other bacterial strains, while its anti-biofilm activity was high against S. aureus. This indicated that ANS exhibited cytotoxic effects on HT29 cells, while it may have less effect on L929. Additionally, molecular docking was used to investigate the in vitro interactions of major compounds with enzymes. The results of binding affinity tests showed that gentisic/salicylic acid adducts had a higher binding affinity than either compound alone.

Graphical Abstract