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<p>This paper aimed to provide a comparative analysis of total phenolic contents (TPC), phytochemical compounds, antioxidant, anti-5-lipoxygenase, anti-xanthine oxidase, and cytotoxic activities of extracts from different organs (leaves, fruits, buds, and stems) of <i>Eucalyptus diversifolia</i>. Extracts were generated using supercritical carbon dioxide (SC-CO₂) extraction, using ethanol as a modifier at a pressure of 9&#xa0;MPa and a temperature of 313&#xa0;K. The TPC (59.9 ± 1.5&#xa0;mg GAE/g DW) of leaf extracts was found to be significantly higher than that of other organs. GC-MS analysis revealed the predominant presence of isocaryophyllene in leaf, bud, and fruit extracts and that of abietic acid in stem extracts. The first reported HPLC screening of extracts was conducted, yielding the identification of twenty phytocompounds. The major compound in leaf and stem extracts was 3′-hydroxy-6-methylflavone, while 3′-hydroxy-b-naphthoflavone was the primary compound in the fruit extract, and rutin was identified as the main compound in the bud extract. The leaf extracts showed the strongest antioxidant and 5-lipoxygenase-inhibiting activities, with IP values of 30.7 and 59.5%, respectively. The bud extracts inhibited the enzyme xanthine oxidase by 24.3%, while other organs were inactive. HCT-116 showed better results compared with the two other cell lines (OVCAR, MCF-7) in all extracts, with the leaf extracts showing a promising inhibition effect (IC<sub>50</sub> = 3.1&#xa0;mg/L).</p>

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Phytochemical Profile and In Vitro Bioactivities of Supercritical Extracts from Various Organs (Leaves, Fruits, Buds, and Stems) of Eucalyptus

  • 期刊论文

  • 发表日期：

    2026年2月18日

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  • Saousan Chamali,
  • Jalloul Bouajila,
  • Severine Camy,
  • Ezzeddine Saadaoui,
  • Houcine Ben Daoud,
  • Jean-Stephane Condoret,
  • Mehrez Romdhane

摘要

This paper aimed to provide a comparative analysis of total phenolic contents (TPC), phytochemical compounds, antioxidant, anti-5-lipoxygenase, anti-xanthine oxidase, and cytotoxic activities of extracts from different organs (leaves, fruits, buds, and stems) of Eucalyptus diversifolia. Extracts were generated using supercritical carbon dioxide (SC-CO₂) extraction, using ethanol as a modifier at a pressure of 9 MPa and a temperature of 313 K. The TPC (59.9 ± 1.5 mg GAE/g DW) of leaf extracts was found to be significantly higher than that of other organs. GC-MS analysis revealed the predominant presence of isocaryophyllene in leaf, bud, and fruit extracts and that of abietic acid in stem extracts. The first reported HPLC screening of extracts was conducted, yielding the identification of twenty phytocompounds. The major compound in leaf and stem extracts was 3′-hydroxy-6-methylflavone, while 3′-hydroxy-b-naphthoflavone was the primary compound in the fruit extract, and rutin was identified as the main compound in the bud extract. The leaf extracts showed the strongest antioxidant and 5-lipoxygenase-inhibiting activities, with IP values of 30.7 and 59.5%, respectively. The bud extracts inhibited the enzyme xanthine oxidase by 24.3%, while other organs were inactive. HCT-116 showed better results compared with the two other cell lines (OVCAR, MCF-7) in all extracts, with the leaf extracts showing a promising inhibition effect (IC50 = 3.1 mg/L).