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<p>The urgent need for precise quantification of a crucial prognostic tumor marker, a human epidermal growth factor receptor 2 (HER2), is of paramount importance for health institutions in screening, diagnosis, and the therapeutic trip of breast cancer. A conspicuous analysis of the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) was conducted using a sandwich electrochemical immunosensing assay on nanomaterial-modified screen-printed carbon electrode (SPCE) surfaces. The SPCEs modified with carboxylated multiwalled carbon nanotubes combined with Fe/Cu bimetallic 1,4-benzenedicarboxylic acid metal-organic frameworks loaded with AgNPs as a sensing platform for carrying capture antibody. The antibody-antigen interaction was identified utilizing horseradish peroxidase (HRP) conjugated detection antibody and 3,3’,5,5’-tetramethylbenzidine (TMB) as the enzymatic substrate. The structural, compositional, and morphological properties of synthesized nanomaterials were characterized by ( FT.IR, XRD, EDX, and FESEM ), before evaluating the analytical performance. The electrochemical signals were obtained by cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry. Linear calibration plots were generated between 20 and 200 pg/mL, and limits of detection (LODs) were identified to be of 5.5 pg/mL, 5.65 pg/mL, and 3.96 pg/mL (for CV, DPV, and SWV), respectively, all much below the defined cut-off value of 15 ng/mL for this cancer biomarker. An immunosensor that had been designed expressly for the purpose of detecting the tumor marker in serum was utilized in order to conduct an experimental evaluation of the procedure’s effectiveness.</p>

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MWCNT Supported by Fe/Cu Bimetallic-based Metal-Organic Frameworks Immobilized with Silver Nanoparticles as a Platform in Electrochemical Immunosensors Fabrication for HER2 Detection in Breast Cancer

  • 期刊论文

  • 发表日期：

    2026年1月29日

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  • Dheyaa Hussein Mohsin,
  • Jassim Mohammed Ali,
  • Tamara N. Ahmed,
  • Saif Jabbar Yasir AL-Mayah

摘要

The urgent need for precise quantification of a crucial prognostic tumor marker, a human epidermal growth factor receptor 2 (HER2), is of paramount importance for health institutions in screening, diagnosis, and the therapeutic trip of breast cancer. A conspicuous analysis of the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) was conducted using a sandwich electrochemical immunosensing assay on nanomaterial-modified screen-printed carbon electrode (SPCE) surfaces. The SPCEs modified with carboxylated multiwalled carbon nanotubes combined with Fe/Cu bimetallic 1,4-benzenedicarboxylic acid metal-organic frameworks loaded with AgNPs as a sensing platform for carrying capture antibody. The antibody-antigen interaction was identified utilizing horseradish peroxidase (HRP) conjugated detection antibody and 3,3’,5,5’-tetramethylbenzidine (TMB) as the enzymatic substrate. The structural, compositional, and morphological properties of synthesized nanomaterials were characterized by ( FT.IR, XRD, EDX, and FESEM ), before evaluating the analytical performance. The electrochemical signals were obtained by cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry. Linear calibration plots were generated between 20 and 200 pg/mL, and limits of detection (LODs) were identified to be of 5.5 pg/mL, 5.65 pg/mL, and 3.96 pg/mL (for CV, DPV, and SWV), respectively, all much below the defined cut-off value of 15 ng/mL for this cancer biomarker. An immunosensor that had been designed expressly for the purpose of detecting the tumor marker in serum was utilized in order to conduct an experimental evaluation of the procedure’s effectiveness.