Purpose <p>The present study describes a first comparative Analysis of supercritical CO₂ extracts and fatty acids profiles from roots and aerial parts of Licorice (<i>Glycyrrhiza glabra</i> L., Fabaceae).</p> Methods <p>The studies were conducted using an experimental setup for secondary metabolites extraction with supercritical carbon dioxide as a promising solvent. The resulting extracts were analyzed via GC–MS technique.</p> Results <p>The quantitatively significant compounds of the aerial parts CO₂ volatile extract were methyl linolenate (12.20%) and n-Hexadecanoic acid (12.04%). However, <i>β</i>-sitosterol (23.26%) followed by n-Hexadecanoic acid were the most abundant secondary metabolites in roots. Moreover, the characterization of Fatty acids composition, revealed a slight variation between aerial parts and roots contents dominated by Palmitic acid accounting for (24.93 and 22.76%, respectively).</p> Conclusion <p>The comparative studies of licorice contents may be valuable in fundamental biological research and demonstrate wide possibilities for the practical application of supercritical fluid technologies aligning with the requirements of “green” chemistry.</p>