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Purpose <p>This study investigated the phytochemical composition and anticancer potential of <i>Clematis heynei</i> saponin-rich fractions against MCF-7 breast cancer cells through integrated chromatographic, spectroscopic, and computational approaches.</p> Methods <p>Authenticated plant material was extracted, fractionated by column chromatography, and characterized using HR-LCMS. Molecular docking against Bcl-2 (PDB: 2W3L) predicted apoptotic interactions, while cytotoxicity was assessed by MTT assay (20–100&#xa0;µg/mL).</p> Results <p>HR-LCMS identified triterpenoids (oleanolic acid, asiatic acid, betulinic acid) in pooled fraction A (PF-A) and flavonoids (quercetin, luteolin, vitexin) in pooled fraction B (PF-B)… with activity comparable to 5-fluorouracil (5-FU) (IC₅₀ = 58.62&#xa0;µg/mL).</p> Conclusion <p><i>Clematis heynei</i> fractions exhibit significant anticancer activity through Bcl-2-mediated apoptosis, warranting further in vivo validation and mechanistic studies for potential development as safer anticancer agents or chemotherapeutic adjuvants.</p> Graphical Abstract <p></p>

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HR-LCMS Guided Phytochemical Profiling and In vitro Anticancer Evaluation of Clematis heynei Fractions Against MCF-7 Cells using MTT Assay with Complementary In silico Docking Analysis

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Sunayana R. Vikhe,
  • Parmeshwar M. Pathade

摘要

Purpose

This study investigated the phytochemical composition and anticancer potential of Clematis heynei saponin-rich fractions against MCF-7 breast cancer cells through integrated chromatographic, spectroscopic, and computational approaches.

Methods

Authenticated plant material was extracted, fractionated by column chromatography, and characterized using HR-LCMS. Molecular docking against Bcl-2 (PDB: 2W3L) predicted apoptotic interactions, while cytotoxicity was assessed by MTT assay (20–100 µg/mL).

Results

HR-LCMS identified triterpenoids (oleanolic acid, asiatic acid, betulinic acid) in pooled fraction A (PF-A) and flavonoids (quercetin, luteolin, vitexin) in pooled fraction B (PF-B)… with activity comparable to 5-fluorouracil (5-FU) (IC₅₀ = 58.62 µg/mL).

Conclusion

Clematis heynei fractions exhibit significant anticancer activity through Bcl-2-mediated apoptosis, warranting further in vivo validation and mechanistic studies for potential development as safer anticancer agents or chemotherapeutic adjuvants.

Graphical Abstract