<p>Mono-cationic pyridinium sulfonamide and mono-cationic quinolinium ionic liquids with varying alkyl chain lengths were successfully synthesized and their pathogenic effects were assessed in a prior attempt. At this juncture, we synthesize mono- and di-sulfonamide of germinal pyridinium and quinolinium ionic liquids and characterize them using NMR, FT-IR, mass, and elemental analysis techniques. The synthesized dicationic sulfonamide ionic liquids were subjected to fundamental structural elucidation studies (FT-IR, NMR, mass and elemental analysis). Due to the presence of sulfonamide and quinolinium groups in the synthesized ILs, biological studies including antioxidation, antibacterial, and antifungal were performed. Compared to mono-sulfonamide ionic liquids, the disulfonamide of pyridinium quinolinium ionic liquid (A5) was shown to have the highest antioxidant activity (IC₅₀-15.36). In addition to its anti-oxidant properties, A5 also had strong antibacterial and antifungal properties.</p>