Scholarlink

<p>Onion basal rot, caused mainly by the soil-borne fungus <i>Fusarium proliferatum</i>, is a serious and persistent disease affecting onion (<i>Allium cepa</i> L.) worldwide. It results in severe yield reductions, impacting both the quantity and quality of the crop. This pathogen penetrates the roots and causes damage to the basal plate of the onion, leading to decay and wilting. It is important to understand how to appropriately manage this disease to minimize its adverse effects. In this study, we examined the metabolite profile and bioactivity of the co-culture system of <i>Aspergillus ochraceus</i> and <i>Penicillium chrysogenum</i> against <i>Fusarium proliferatum</i>, one of the main pathogens responsible for onion basal rot. By employing integrative analytical techniques such as GC-MS and LC-MS/MS, the metabolite profile of the co-culture extract was identified, particularly in the dichloromethane fractions. The fraction demonstrated the highest antifungal activity, with a minimum inhibitory concentration (MIC) of 0.39&#xa0;mg/mL and exhibited the lowest cytotoxicity in the brine shrimp assay. A molecular docking study on bioactive chemicals revealed that, among others, spirobrocazine A, palmitic acid, spathullin B, and citridone H demonstrated a very high affinity for the cutinase enzyme from <i>F. proliferatum</i>, possibly leading to inhibitory mechanisms. The results demonstrated that fungal co-cultures have the potential to produce bioactive compounds that could serve in the production of biocontrol products with an eco-friendly application to counteract <i>Fusarium</i> infections in plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metabolites Profiling and Bioactivity of Aspergillus-Penicillium Co-culture: Integrative GC-MS, LC-MS/MS, and in Silico Analysis for Targeting Fusarium Proliferatum in Onion Basal Rot Biocontrol

  • 期刊论文

  • 发表日期：

    2025年9月3日

查看全文
  • Mohammed M. M. Abdelrahem,
  • Abdallah M. A. Hassane,
  • Nageh F. Abo-Dahab,
  • Mohamed E. Abouelela

摘要

Onion basal rot, caused mainly by the soil-borne fungus Fusarium proliferatum, is a serious and persistent disease affecting onion (Allium cepa L.) worldwide. It results in severe yield reductions, impacting both the quantity and quality of the crop. This pathogen penetrates the roots and causes damage to the basal plate of the onion, leading to decay and wilting. It is important to understand how to appropriately manage this disease to minimize its adverse effects. In this study, we examined the metabolite profile and bioactivity of the co-culture system of Aspergillus ochraceus and Penicillium chrysogenum against Fusarium proliferatum, one of the main pathogens responsible for onion basal rot. By employing integrative analytical techniques such as GC-MS and LC-MS/MS, the metabolite profile of the co-culture extract was identified, particularly in the dichloromethane fractions. The fraction demonstrated the highest antifungal activity, with a minimum inhibitory concentration (MIC) of 0.39 mg/mL and exhibited the lowest cytotoxicity in the brine shrimp assay. A molecular docking study on bioactive chemicals revealed that, among others, spirobrocazine A, palmitic acid, spathullin B, and citridone H demonstrated a very high affinity for the cutinase enzyme from F. proliferatum, possibly leading to inhibitory mechanisms. The results demonstrated that fungal co-cultures have the potential to produce bioactive compounds that could serve in the production of biocontrol products with an eco-friendly application to counteract Fusarium infections in plants.