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<p>To produce valuable fermented products, microbial growth requires different microbial cultures, which can be expensive and readily unavailable worldwide. So, developing countries spend large amounts of money to import such microbial media. In the backdrop of this issue, there’s a need for alternative microbial growth media that can reduce cost and are easily available. Sericin is an industrial waste that pollutes the ecosystem and is unfriendly to the environment, composed of protein that contains huge amino acids quantity and other nutrients and can act as an alternative to microbial culture media. Optimization of sericin is the key step in its utilization as microbe culture media. Various variables, such as the concentration of sericin, incubation time, and pH were optimized to obtain the maximum microbial growth through Response Surface Methodology (RSM). Sericin was extracted from silkworms. After the extraction, the pH and concentration of sericin were changed, and the <i>E. coli</i> were spread on the sericin media. The plates were incubated, and the bacterial colonies were counted at different incubation times. The results showed that the bacteria grew at neutral pH when the concentration of sericin and the incubation period were high.</p>

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Sericin as Novel Microbial Culture Medium: Proportion Optimization using Response Surface Methodology (RSM) Towards Environmental Health Improvement

  • 期刊论文

  • 发表日期：

    2025年8月5日

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  • Saima Shahzad Mirza,
  • Sibtain Ahmed,
  • Yamina Khalid,
  • Hafiz Muhammad Tahir,
  • Ayesha Ishaque,
  • Khayala Mammadova,
  • Khalid Fahmi Fawy,
  • Muhammad Arshad

摘要

To produce valuable fermented products, microbial growth requires different microbial cultures, which can be expensive and readily unavailable worldwide. So, developing countries spend large amounts of money to import such microbial media. In the backdrop of this issue, there’s a need for alternative microbial growth media that can reduce cost and are easily available. Sericin is an industrial waste that pollutes the ecosystem and is unfriendly to the environment, composed of protein that contains huge amino acids quantity and other nutrients and can act as an alternative to microbial culture media. Optimization of sericin is the key step in its utilization as microbe culture media. Various variables, such as the concentration of sericin, incubation time, and pH were optimized to obtain the maximum microbial growth through Response Surface Methodology (RSM). Sericin was extracted from silkworms. After the extraction, the pH and concentration of sericin were changed, and the E. coli were spread on the sericin media. The plates were incubated, and the bacterial colonies were counted at different incubation times. The results showed that the bacteria grew at neutral pH when the concentration of sericin and the incubation period were high.