<p>Since the innovation produces a significant output with minimal effort, the environmentally friendly method of metal oxide creation presents a viable alternative to the more labor-intensive and difficult-to-consume predecessors processes. This paper describes the sustainable creation of TiO₂ nanoparticles (NP_S) using feedstock from <i>Allium sativum</i> (Garlic). The synthesized TiO₂ NP_S is thoroughly characterized using novel approaches, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FE-SEM). The images from XRD indicate the anatase condition of the TiO₂ NP_S. The FT-IR spectrum endorses the Ti–O vibratory patterns at 787&#xa0;cm⁻¹. The geometrical features of the synthesized TiO₂ NP_S have been demonstrated in the FE-SEM illustrations, revealing that agglomerate occurs in the particles that are synthesized owing to the interactions within the substances of the feedstock. The morphological evaluation indicated that the NP_S exhibited a sphere-like morphology, exhibiting an average dimension between 18 and 24&#xa0;nm. The antibacterial efficacy of TiO₂ NP_S was evaluated employing the established agar-well dissemination technique, revealing a greater zone of inhibition against <i>S. epidermidis</i> and <i>S. aureus</i> compared to <i>E. coli</i> and <i>F. nucleatum</i>. Additionally, the NP_S demonstrated notable cellular damage towards the people breast carcinoma cell lines (HepG2), with an IC₅₀ value of 54.66&#xa0;µg/mL. The research investigated the photodecomposition efficacy of TiO₂ NP_S in an aqueous solution, concentrating on the MB dye, following direct light transmission at varying duration periods. The TiO₂ NP_S generated by creation exhibited significant photodecomposition efficiency, degrading 88% in 70&#xa0;min. The findings reveal that the garlic-based creation of TiO₂ NP_S is straightforward, environmentally benign, and has versatility capabilities.</p> Graphical Abstract <p></p>