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<p>Microalgae are a promising and renewable source of fatty acids, capable of accumulating lipids and improving fatty acid quality under various stress conditions. This study investigated the effects of carbon enrichment on lipid production and fatty acid content in <i>Micractinium pusillum</i> using different carbon sources. <i>M. pusillum</i> showed efficient absorption and metabolism of 3 g/L sodium acetate, while glycerol and glucose were less effective. The optimal concentration of 3 g/L sodium acetate increased lipid productivity by 95% and biomass by 62%. GC–MS analysis indicated a higher omega-3 content in the lipid extract from the 3 g/L sodium acetate culture than the control, suggesting that carbon enrichment significantly influences the fatty acid profile. The research also assessed the antioxidant and cytotoxic properties of the produced lipids. The lipid extract from the 3 g/L sodium acetate culture demonstrated substantial antioxidant capacity, achieving 70.17% DPPH* scavenging activity at 100 μg/mL. Cytotoxicity studies revealed significant cytotoxic effects on HepG2 and MCF-7 cell lines (IC50 = 18.21 and 15.56 μg/mL, respectively) and notable activity against the HCT-116 cell line (IC50 = 43.37 μg/mL). In contrast, the lipid extract from the <i>M. pusillum</i> control culture exhibited only modest cytotoxic effects across all tested cell lines. This research highlights a novel approach for enhancing the production of omega-3 LC-PUFAs from <i>M. pusillum</i> through carbon enrichment. The lipid extracts show significant biological properties, including antioxidant and anticancer activities, emphasizing their potential applications in the nutraceutical and therapeutic industries.</p>

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The Impact of Various Carbon Sources on Lipid Production in Cultivated Micractinium pusillum and the Evaluation of Antioxidant and Antiproliferative Properties of the Most Omega-3-Rich Lipid Extract

  • 期刊论文

  • 发表日期：

    2025年3月5日

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  • Mostafa M. El-Sheekh,
  • Heba M. Mansour,
  • Mohamed Y. Bedaiwy,
  • Rania A. El-shenody

摘要

Microalgae are a promising and renewable source of fatty acids, capable of accumulating lipids and improving fatty acid quality under various stress conditions. This study investigated the effects of carbon enrichment on lipid production and fatty acid content in Micractinium pusillum using different carbon sources. M. pusillum showed efficient absorption and metabolism of 3 g/L sodium acetate, while glycerol and glucose were less effective. The optimal concentration of 3 g/L sodium acetate increased lipid productivity by 95% and biomass by 62%. GC–MS analysis indicated a higher omega-3 content in the lipid extract from the 3 g/L sodium acetate culture than the control, suggesting that carbon enrichment significantly influences the fatty acid profile. The research also assessed the antioxidant and cytotoxic properties of the produced lipids. The lipid extract from the 3 g/L sodium acetate culture demonstrated substantial antioxidant capacity, achieving 70.17% DPPH* scavenging activity at 100 μg/mL. Cytotoxicity studies revealed significant cytotoxic effects on HepG2 and MCF-7 cell lines (IC50 = 18.21 and 15.56 μg/mL, respectively) and notable activity against the HCT-116 cell line (IC50 = 43.37 μg/mL). In contrast, the lipid extract from the M. pusillum control culture exhibited only modest cytotoxic effects across all tested cell lines. This research highlights a novel approach for enhancing the production of omega-3 LC-PUFAs from M. pusillum through carbon enrichment. The lipid extracts show significant biological properties, including antioxidant and anticancer activities, emphasizing their potential applications in the nutraceutical and therapeutic industries.