Assessment Defeating of Breast Cancer MCF-7 Cells, and Bacterial Species by Spherical Gold Nanoparticles Fabricated Through Reductive Ability of Framed Bio-Organic Molecules

Breast cancer patients continue to suffer from the side effects of chemotherapies, which are toxic and are not well absorbed. Thus, non-toxic and effective treatment approaches need to be developed, particularly green synthesizing gold nanoparticles (AuNPs). The study was intended for bio-preparation of AuNPs mediated by reductive ability agent as rich in OH groups away from any chemical method, characterized as a spherical shape, small size (22.5 nm), negative surface charge, also stable in colloidal solution with highest absorption at 550 nm. AuNPs noticed penetrate MCF-7 cells rely on MTT cytotoxicity test in (IC₅₀) at 350 µg/mL. AuNPs appeared capable of suppressing bacterial colonies in small concentrations in assessing antibiotic drugs. AuNPs also possess a genotoxicity feature illustrated in DNA population by cell cycle technique in diminishing G1 and S-phase due to rising apoptosis in treated cells. In addition to emphasizing genotoxicity via the comet assay, the percentage of cancer cells annihilated in alkaline and neutral conditions is 33% and 41%, respectively. AuNPs are considered a governing agent in the breast cancer field.