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<p>The study emphasizes an in-depth in <i>vitro</i> efficiency analysis of bacterial cellulose (BC)-polyvinylpyrrolidone (PVP) composite hydrogel scaffolds which are doped with different concentrations of β-tri-calcium phosphate (β-TCP) and hydroxyapatite (HA). The hydrogels are prepared with the natural polymer, BC, and synthetic polymer, PVP. Polyethylene glycol (PEG) is also used as an anti-cytotoxic agent and agar is used as a gelling agent. β-TCP and HA were given in the ratio (w/w): 20:80 (BC-PVP- β-TCP/HA_20:80), 40:60 (BC-PVP-β-TCP/HA_40:60), 50:50 (BC-PVP-β-TCP/HA_50:50). In vitro efficacy of the different tri-calcium phosphate (β-TCP) and hydroxyapatite BC-PVP composite hydrogel scaffolds are analyzed through comparative analysis of the cell viability with human osteosarcoma cell line, Saos-2 and human adipose derived mesenchymal stem cells (hMSC). Notable cell viability is found with the scaffolds. Additionally, Ki-67 protein expression confirmed the cell proliferation without any significant cyto-pathological effects. Furthermore, significant expression of alkaline phosphatase (ALP) was observed with BC-PVP- β-TCP/HA_20:80 and BC-PVP- β-TCP/HA_50:50 scaffolds. Finally, SEM study indicated the notable adhesion and proliferation of Saos-2 on to the surface of the hydrogel scaffolds. Thus, these studies indicate the in vitro effectiveness of different tri-calcium phosphate (β-TCP) and hydroxyapatite doped BC-PVP composite hydrogel scaffolds and therefore recommended for further studies for bone tissue repair application.</p>

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In vitro efficiency analysis of different β-tri-calcium phosphate and hydroxyapatite doped bacterial cellulose-polyvinylpyrrolidone composite hydrogel for bone tissue repair

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月16日

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  • Probal Basu,
  • Nabanita Saha,
  • Radostina Alexandrova,
  • Boika Andonova Lilova,
  • Maya Argirova,
  • Petr Saha

摘要

The study emphasizes an in-depth in vitro efficiency analysis of bacterial cellulose (BC)-polyvinylpyrrolidone (PVP) composite hydrogel scaffolds which are doped with different concentrations of β-tri-calcium phosphate (β-TCP) and hydroxyapatite (HA). The hydrogels are prepared with the natural polymer, BC, and synthetic polymer, PVP. Polyethylene glycol (PEG) is also used as an anti-cytotoxic agent and agar is used as a gelling agent. β-TCP and HA were given in the ratio (w/w): 20:80 (BC-PVP- β-TCP/HA_20:80), 40:60 (BC-PVP-β-TCP/HA_40:60), 50:50 (BC-PVP-β-TCP/HA_50:50). In vitro efficacy of the different tri-calcium phosphate (β-TCP) and hydroxyapatite BC-PVP composite hydrogel scaffolds are analyzed through comparative analysis of the cell viability with human osteosarcoma cell line, Saos-2 and human adipose derived mesenchymal stem cells (hMSC). Notable cell viability is found with the scaffolds. Additionally, Ki-67 protein expression confirmed the cell proliferation without any significant cyto-pathological effects. Furthermore, significant expression of alkaline phosphatase (ALP) was observed with BC-PVP- β-TCP/HA_20:80 and BC-PVP- β-TCP/HA_50:50 scaffolds. Finally, SEM study indicated the notable adhesion and proliferation of Saos-2 on to the surface of the hydrogel scaffolds. Thus, these studies indicate the in vitro effectiveness of different tri-calcium phosphate (β-TCP) and hydroxyapatite doped BC-PVP composite hydrogel scaffolds and therefore recommended for further studies for bone tissue repair application.