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<p>To identify the pathogen causing chlorosis symptoms in aconitum (<i>Aconitum carmichaelii </i>Debx<i>.</i>), the complete genome sequence of aconitum virus 2 Hebei isolate (AcV2-Hebei) was determined through deep sequencing of a small RNA library derived from AcV2-infected samples, complemented by Rapid Amplification of cDNA Ends (RACE) PCR. The full-length genome of the AcV2-Hebei is 9,455 nt. Sequence alignment revealed remarkable homology, with nucleotide and amino acid sequence identities of 92.82% and 97.03%, respectively, between AcV2-Hebei and the previously reported Yunnan isolate. Molecular diversity was predominantly observed in the P1 and CP regions, highlighting potential functional or structural variations. Phylogenetic analysis revealed that the AcV2-Hebei clusters closely with the AcV2 Yunnan isolate and shares a close evolutionary relationship with lily mottle virus, lily virus A, and tulip breaking virus. This study marks the first report of a complete genome sequence for an AcV2 isolate from northern China. It not only enriches the existing AcV2 sequence database but also provides a robust molecular foundation for subsequent investigations into the biological characteristics and pathogenicity of AcV2.</p>

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Identification and complete genome sequence analysis of aconitum virus 2 from Hebei infecting Aconitum carmichaelii Debx

  • 期刊论文

  • 发表日期：

    2026年6月24日

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  • Hui Chen,
  • Lu Hao

摘要

To identify the pathogen causing chlorosis symptoms in aconitum (Aconitum carmichaelii Debx.), the complete genome sequence of aconitum virus 2 Hebei isolate (AcV2-Hebei) was determined through deep sequencing of a small RNA library derived from AcV2-infected samples, complemented by Rapid Amplification of cDNA Ends (RACE) PCR. The full-length genome of the AcV2-Hebei is 9,455 nt. Sequence alignment revealed remarkable homology, with nucleotide and amino acid sequence identities of 92.82% and 97.03%, respectively, between AcV2-Hebei and the previously reported Yunnan isolate. Molecular diversity was predominantly observed in the P1 and CP regions, highlighting potential functional or structural variations. Phylogenetic analysis revealed that the AcV2-Hebei clusters closely with the AcV2 Yunnan isolate and shares a close evolutionary relationship with lily mottle virus, lily virus A, and tulip breaking virus. This study marks the first report of a complete genome sequence for an AcV2 isolate from northern China. It not only enriches the existing AcV2 sequence database but also provides a robust molecular foundation for subsequent investigations into the biological characteristics and pathogenicity of AcV2.