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<p>In the winter of 2019, stem rot disease was observed in a commercial camelina (<i>Camelina sativa</i>) field in Gorgan, northern Iran. The causal agent was identified as <i>Sclerotinia sclerotiorum</i> using classical and molecular approaches. Subsequently, 133 advanced double-haploid genotypes were evaluated for resistance to this disease in the field over two consecutive years (2020–2021 and 2021–2022) and under greenhouse conditions. Plants were inoculated by making a stem incision and placing a fresh fungal colony plug at the site. Disease reaction was assessed by measuring the length of necrotic or chlorotic lesions, the number and length of produced sclerotia, and the mortality percentage. Field results showed that 6 (4.51%), 88 (56.16%), 36 (17.06%), and 3 (2.25%) genotypes were resistant, moderately resistant, moderately susceptible, and susceptible, respectively. Greenhouse testing revealed that the genotype × isolate interaction was significant for all measured factors, indicating variation in aggressiveness among isolates. Furthermore, the distribution pattern of genotypes based on necrosis length suggested that the resistance is horizontal in nature. Based on combined field and greenhouse results, seven genotypes (24, 54, 59, 60, 132, 133, and 137) were identified as moderately to highly resistant. These genotypes are recommended as resistance sources for breeding programs or for release as new cultivars following complementary agronomic trials.</p>

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Screening double-haploid germplasm of camelina for resistance to sclerotinia stem rot in the Caspian Sea zone of Iran

  • 期刊论文

  • 发表日期：

    2026年5月21日

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  • Hamid Reza Pouralibaba,
  • Shahriyar Kia

摘要

In the winter of 2019, stem rot disease was observed in a commercial camelina (Camelina sativa) field in Gorgan, northern Iran. The causal agent was identified as Sclerotinia sclerotiorum using classical and molecular approaches. Subsequently, 133 advanced double-haploid genotypes were evaluated for resistance to this disease in the field over two consecutive years (2020–2021 and 2021–2022) and under greenhouse conditions. Plants were inoculated by making a stem incision and placing a fresh fungal colony plug at the site. Disease reaction was assessed by measuring the length of necrotic or chlorotic lesions, the number and length of produced sclerotia, and the mortality percentage. Field results showed that 6 (4.51%), 88 (56.16%), 36 (17.06%), and 3 (2.25%) genotypes were resistant, moderately resistant, moderately susceptible, and susceptible, respectively. Greenhouse testing revealed that the genotype × isolate interaction was significant for all measured factors, indicating variation in aggressiveness among isolates. Furthermore, the distribution pattern of genotypes based on necrosis length suggested that the resistance is horizontal in nature. Based on combined field and greenhouse results, seven genotypes (24, 54, 59, 60, 132, 133, and 137) were identified as moderately to highly resistant. These genotypes are recommended as resistance sources for breeding programs or for release as new cultivars following complementary agronomic trials.