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<p>High-throughput sequencing (HTS) analysis of sweet cherry (<i>Prunus avium</i>) plants from an autochthonous germplasm collection in Locorotondo (Apulia, Italy) led to the identification of two novel isolates of pomes virus Greece (PVGR), belonging to the genus <i>Robigovirus</i>. These isolates, designated PVGR IT-49 and IT-80 (accession numbers PX657514 and PX657515, respectively), represent the first report of PVGR in cherry and the first detection of this virus in Italy. Specific and degenerate primers for <i>Robigovirus</i> detection were developed and validated through Sanger sequencing. Their application to 150 samples collected across three sites in Apulia revealed a PVGR prevalence of 8.7% (13 samples). In addition, one tree was found infected with cherry green ring mottle virus (CGRMV), which constitutes the first report of this robigovirus in Apulia. Comparative analysis of 13 partial PVGR nucleotide sequences showed 79–83.5% identity with the previously reported Greek isolate from pear. Biological indexing of eight local PVGR isolates confirmed their pathogenicity, inducing symptoms such as leaf crinkling and necrotic spotting on the Bing and Shirofugen indicators. Overall, this study expands the list of viruses known to infect cherry trees and documents for the first time the occurrence of robigoviruses in Apulia region.</p>

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Pomes virus Greece (PVGR): a new robigovirus infecting cherry trees in Apulia (southern Italy)

  • 期刊论文

  • 发表日期：

    2026年4月14日

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  • Michele Digiaro,
  • Arwa Amleh,
  • Pasquale Venerito,
  • Amani Ben Slimen

摘要

High-throughput sequencing (HTS) analysis of sweet cherry (Prunus avium) plants from an autochthonous germplasm collection in Locorotondo (Apulia, Italy) led to the identification of two novel isolates of pomes virus Greece (PVGR), belonging to the genus Robigovirus. These isolates, designated PVGR IT-49 and IT-80 (accession numbers PX657514 and PX657515, respectively), represent the first report of PVGR in cherry and the first detection of this virus in Italy. Specific and degenerate primers for Robigovirus detection were developed and validated through Sanger sequencing. Their application to 150 samples collected across three sites in Apulia revealed a PVGR prevalence of 8.7% (13 samples). In addition, one tree was found infected with cherry green ring mottle virus (CGRMV), which constitutes the first report of this robigovirus in Apulia. Comparative analysis of 13 partial PVGR nucleotide sequences showed 79–83.5% identity with the previously reported Greek isolate from pear. Biological indexing of eight local PVGR isolates confirmed their pathogenicity, inducing symptoms such as leaf crinkling and necrotic spotting on the Bing and Shirofugen indicators. Overall, this study expands the list of viruses known to infect cherry trees and documents for the first time the occurrence of robigoviruses in Apulia region.