Scholarlink

<p>The infection of host plants by specific pathogens plays a crucial role in shaping and maintaining biodiversity within forest ecosystems. However, the molecular mechanisms underlying the interactions between pathogens and host plants remain poorly understood. In this study, we explored the metabolic interactions between the endophytic pathogen <i>Endomelanconiopsis endophytica</i> and its host tree, <i>Castanopsis fissa</i>, in a subtropical forest. During the infection process, we identified 63 metabolites from <i>E. endophytica</i> and 89 metabolites from <i>C. fissa</i>. Key metabolites, such as β-alanine (FC = 3.5, <i>p</i> &lt; 0.01) and oleic acid (C18:1, FC = 4.7, <i>p</i> &lt; 0.01), were found to be essential mediators. <i>E. endophytica</i> upregulated amino acid and fatty acid metabolism throughout the infection, with continued pathway activation after 24&#xa0;h post-inoculation (hpi). Conversely, <i>C. fissa</i> displayed inhibited energy and amino acid metabolism. As a defense response, it activated fructose and mannose metabolism. Our study presents the first metabolic roadmap of a Botryosphaeriales pathogen’s host-specific strategy. These findings offer molecular-level insights into the maintenance of biodiversity in subtropical forests.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metabolic shift and signature metabolites unveiled during Endomelanconiopsis endophytica infection of Castanopsis fissa

  • 期刊论文

  • 发表日期：

    2026年3月17日

查看全文
  • Liuqing Shi,
  • Yan Xie,
  • Yang Li,
  • Pingyu Tan,
  • Minxia Liang,
  • Xubing Liu,
  • Shixiao Yu

摘要

The infection of host plants by specific pathogens plays a crucial role in shaping and maintaining biodiversity within forest ecosystems. However, the molecular mechanisms underlying the interactions between pathogens and host plants remain poorly understood. In this study, we explored the metabolic interactions between the endophytic pathogen Endomelanconiopsis endophytica and its host tree, Castanopsis fissa, in a subtropical forest. During the infection process, we identified 63 metabolites from E. endophytica and 89 metabolites from C. fissa. Key metabolites, such as β-alanine (FC = 3.5, p < 0.01) and oleic acid (C18:1, FC = 4.7, p < 0.01), were found to be essential mediators. E. endophytica upregulated amino acid and fatty acid metabolism throughout the infection, with continued pathway activation after 24 h post-inoculation (hpi). Conversely, C. fissa displayed inhibited energy and amino acid metabolism. As a defense response, it activated fructose and mannose metabolism. Our study presents the first metabolic roadmap of a Botryosphaeriales pathogen’s host-specific strategy. These findings offer molecular-level insights into the maintenance of biodiversity in subtropical forests.