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<p>Chilli yellow ringspot virus (CYRSV, genus <i>Orthotospovirus</i>, family <i>Tospoviridae</i>) can cause diseases in pepper and tomato that affect the yield and quality of fruits, with symptoms such as tomato fruit yellowing, ring spots, wrinkles, and necrosis. However, the complete genome sequence of a CYRSV isolate from tomato is not determined yet. Here, we present the complete genome sequence of a CYRSV isolate from a tomato plant (CYRSV-21YV081 isolate) from Yunnan Province, China. The genome of CYRSV-21YV081 contained 3424, 4840, and 8917 nt of small (S), medium (M), and large (L) RNA segments, respectively. Analysis of the whole-genome sequence of CYRSV revealed the genomic characteristics of members of the genus <i>Orthotospovirus</i>. Comparative analysis of the complete S, M and L RNA nucleotide sequences of CYRSV-21YV081 and a CYRSV pepper isolate (CYRSV-14YV855) revealed 99.04%, 89.05%, and 98.97% nucleotide identity, respectively. The region with the greatest difference in the whole-genome sequences of CYRSV-21YV081 and CYRSV-14YV855 is the intergenic region of the M RNA segment, with only 69.64% homology. Further comparative analysis of the amino acid homology of viral proteins revealed that the sequences of the nucleocapsid (N) protein, S RNA-encoded nonstructural (NSs) protein, M RNA-encoded nonstructural (NSm) protein, Gn/Gc glycoprotein precursor, and RNA-dependent RNA polymerase (RdRp) protein were 99.64%, 99.32%, 94.14%, 95.99% and 99.34% identical, respectively, to those of CYRSV isolate 14YV855. Phylogenetic analysis revealed that the N, NSs, NSm, Gn/Gc and RdRp proteins of CYRSV-21YV081 clustered with those of the CYRSV-14YV855 isolate. The full sequence of CYRSV-21YV081 provides a reference genome for comparison with that of other orthotospovirus.</p>

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The characterization and complete genome sequence of a new strain of chilli yellow ringspot virus infecting tomato in China

  • 期刊论文

  • 发表日期：

    2026年3月5日

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  • Xiaoqin Li,
  • Ruyi Li,
  • Kuo Wu,
  • Yongdui Chen,
  • Zhongkai Zhang,
  • Yu Li

摘要

Chilli yellow ringspot virus (CYRSV, genus Orthotospovirus, family Tospoviridae) can cause diseases in pepper and tomato that affect the yield and quality of fruits, with symptoms such as tomato fruit yellowing, ring spots, wrinkles, and necrosis. However, the complete genome sequence of a CYRSV isolate from tomato is not determined yet. Here, we present the complete genome sequence of a CYRSV isolate from a tomato plant (CYRSV-21YV081 isolate) from Yunnan Province, China. The genome of CYRSV-21YV081 contained 3424, 4840, and 8917 nt of small (S), medium (M), and large (L) RNA segments, respectively. Analysis of the whole-genome sequence of CYRSV revealed the genomic characteristics of members of the genus Orthotospovirus. Comparative analysis of the complete S, M and L RNA nucleotide sequences of CYRSV-21YV081 and a CYRSV pepper isolate (CYRSV-14YV855) revealed 99.04%, 89.05%, and 98.97% nucleotide identity, respectively. The region with the greatest difference in the whole-genome sequences of CYRSV-21YV081 and CYRSV-14YV855 is the intergenic region of the M RNA segment, with only 69.64% homology. Further comparative analysis of the amino acid homology of viral proteins revealed that the sequences of the nucleocapsid (N) protein, S RNA-encoded nonstructural (NSs) protein, M RNA-encoded nonstructural (NSm) protein, Gn/Gc glycoprotein precursor, and RNA-dependent RNA polymerase (RdRp) protein were 99.64%, 99.32%, 94.14%, 95.99% and 99.34% identical, respectively, to those of CYRSV isolate 14YV855. Phylogenetic analysis revealed that the N, NSs, NSm, Gn/Gc and RdRp proteins of CYRSV-21YV081 clustered with those of the CYRSV-14YV855 isolate. The full sequence of CYRSV-21YV081 provides a reference genome for comparison with that of other orthotospovirus.