Scholarlink

<p>A novel idaeovirus was discovered in stinging nettles (<i>Urtica dioica</i>) with yellow mosaics on the leaves. The virus was initially detected in the Main Botanical Garden, Moscow, Russia, using high-throughput sequencing and was tentatively named “nettle idaeovirus” (NIdV). The complete NIdV genomes from two symptomatic plants (designated as YM and VB) consisted of two RNAs of 5424 and 2195 nucleotides (nt) that were predicted to encode typical idaeovirus proteins. NIdV-YM and NIdV-VB isolates shared 98.6% (RNA1) and 99.6% (RNA2) nt identity and were most closely related to black mulberry idaeovirus with 69.4% and 76.5% nt identity for RNA1 and RNA2, respectively. Nettles with yellow mosaics were also found in other locations in Moscow and the surrounding area, tens of kilometers apart. All these samples tested positive for NIdV using RT-PCR with primers designed based on the complete NIdV genome sequences. The PCR product sequences of NIdV isolates from different locations had an average nt identity of 98.8%. Based on the genome structure, sequence identity with known idaeoviruses and phylogenetic analysis, NIdV can be considered a new putative member of the genus <i>Idaeovirus</i>. NIdV finding in nettles has added to the list of natural hosts for idaeoviruses and to the list of viruses infecting nettle.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Discovery and complete genome characterization of a novel idaeovirus from nettle (Urtica dioica)

  • 期刊论文

  • 发表日期：

    2026年2月19日

查看全文
  • Elena Motsar,
  • Anna Sheveleva,
  • Svetlana Tsygankova,
  • Fedor Sharko,
  • Natalia Slobodova,
  • Irina Mitrofanova,
  • Sergei Chirkov

摘要

A novel idaeovirus was discovered in stinging nettles (Urtica dioica) with yellow mosaics on the leaves. The virus was initially detected in the Main Botanical Garden, Moscow, Russia, using high-throughput sequencing and was tentatively named “nettle idaeovirus” (NIdV). The complete NIdV genomes from two symptomatic plants (designated as YM and VB) consisted of two RNAs of 5424 and 2195 nucleotides (nt) that were predicted to encode typical idaeovirus proteins. NIdV-YM and NIdV-VB isolates shared 98.6% (RNA1) and 99.6% (RNA2) nt identity and were most closely related to black mulberry idaeovirus with 69.4% and 76.5% nt identity for RNA1 and RNA2, respectively. Nettles with yellow mosaics were also found in other locations in Moscow and the surrounding area, tens of kilometers apart. All these samples tested positive for NIdV using RT-PCR with primers designed based on the complete NIdV genome sequences. The PCR product sequences of NIdV isolates from different locations had an average nt identity of 98.8%. Based on the genome structure, sequence identity with known idaeoviruses and phylogenetic analysis, NIdV can be considered a new putative member of the genus Idaeovirus. NIdV finding in nettles has added to the list of natural hosts for idaeoviruses and to the list of viruses infecting nettle.