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<p>Cotton, one of the most important fibre crops in the Indian subcontinent, has been severely affected by cotton leaf curl disease (CLCuD) for the past three decades. Developing and using CLCuD-resistant cotton varieties is the only long-term, environmentally friendly solution to protect the crop from major losses. In this study, two plant types of an exotic cotton accession, Mac-07 [Mac-07 small leaf (Mac-07SL) and Mac-07 broad leaf (Mac-07BL)], along with a susceptible variety, V-5, were tested against CLCuD under high inoculums pressure in the field, as well as through artificial inoculation using whitefly transmission and grafting under controlled conditions. The source plants used for artificial inoculation carried a mix of viruses [cotton leaf curl Multan virus (CLCuMuV) and cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu)], which were involved in first and second epidemics of CLCuD in cotton crop in Pakistan. They also contained single type of betasatellite (CLCuMuB) and six different types of alphasatellites. The present results showed that Mac-07SL cotton is highly resistant to CLCuD in field conditions and cotton leaf curl Multan virus-Rajasthan (CLCuMuV-Raj) strain is the most common virus linked to CLCuD in the field. However, results from the artificial inoculation studies showed that Mac-07SL, Mac-07BL and V5 were all prone to infection, but Mac-07SL showed the higher level of resistance. Molecular analysis revealed that artificially inoculated Mac-07SL plants contained only CLCuMuV, Mac-07BL and V5 plants contained both CLCuMuV and CLCuKoV-Bu. In the double-graft inoculation assay, it was observed that the replication of CLCuKoV-Bu was significantly attenuated in Mac-07SL. However, both viruses (CLCuMuV and CLCuKoV-Bu), the betasatellite (CLCuMuB) and five type of alphasatellites were still able to move through the phloem of Mac-07SL into the second grafts of the susceptible genotype V5. Overall, the results from all testing methods confirm that the exotic genotype Mac-07SL is a strong source of resistance against CLCuD-complex.</p>

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Evaluation of resistance level of exotic cotton Gossypium hirsutum accession “Mac-07” against cotton leaf curl disease complex

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Khalid Pervaiz Akhtar,
  • Sajawal Iqbal,
  • Najeeb Ullah,
  • Muhammad Qasim Aslam,
  • Muhammad Kashif Riaz Khan,
  • Imran Amin

摘要

Cotton, one of the most important fibre crops in the Indian subcontinent, has been severely affected by cotton leaf curl disease (CLCuD) for the past three decades. Developing and using CLCuD-resistant cotton varieties is the only long-term, environmentally friendly solution to protect the crop from major losses. In this study, two plant types of an exotic cotton accession, Mac-07 [Mac-07 small leaf (Mac-07SL) and Mac-07 broad leaf (Mac-07BL)], along with a susceptible variety, V-5, were tested against CLCuD under high inoculums pressure in the field, as well as through artificial inoculation using whitefly transmission and grafting under controlled conditions. The source plants used for artificial inoculation carried a mix of viruses [cotton leaf curl Multan virus (CLCuMuV) and cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu)], which were involved in first and second epidemics of CLCuD in cotton crop in Pakistan. They also contained single type of betasatellite (CLCuMuB) and six different types of alphasatellites. The present results showed that Mac-07SL cotton is highly resistant to CLCuD in field conditions and cotton leaf curl Multan virus-Rajasthan (CLCuMuV-Raj) strain is the most common virus linked to CLCuD in the field. However, results from the artificial inoculation studies showed that Mac-07SL, Mac-07BL and V5 were all prone to infection, but Mac-07SL showed the higher level of resistance. Molecular analysis revealed that artificially inoculated Mac-07SL plants contained only CLCuMuV, Mac-07BL and V5 plants contained both CLCuMuV and CLCuKoV-Bu. In the double-graft inoculation assay, it was observed that the replication of CLCuKoV-Bu was significantly attenuated in Mac-07SL. However, both viruses (CLCuMuV and CLCuKoV-Bu), the betasatellite (CLCuMuB) and five type of alphasatellites were still able to move through the phloem of Mac-07SL into the second grafts of the susceptible genotype V5. Overall, the results from all testing methods confirm that the exotic genotype Mac-07SL is a strong source of resistance against CLCuD-complex.