<p>Take-All disease is one of the most significant soil-borne diseases affecting wheat, leading to reduced yield and quality. This study aimed to investigate the role of lignification and the effect of nutrients on resistance to Take-All disease. To achieve this, six bread wheat genotypes (164, 1526, 1528, 1546, 1622, and 1879) and two wild wheat relatives (<i>Aegilops</i> and <i>Boeticum</i>) were evaluated in the experimental field of the Agricultural Faculty at Vali-e-Asr University of Rafsanjan. The measured traits included the lignin content of the crown and the nutrient levels in the crown tissue. The results indicated that resistant genotypes exhibited higher lignin levels compared to susceptible ones, suggesting a role for lignin in providing structural resistance against the disease. In addition, resistant genotypes exhibited elevated levels of potassium, manganese, and iron, which are involved in lignin synthesis and the activation of plant defense pathways. The resistance genotypes 1879 and 1528 exhibited 4.6% and 4.1% higher lignin content, respectively, compared to susceptible genotypes. This study showed that lignification and adequate supply of key nutrients play a crucial role in reducing the severity of take-all disease and improving wheat resistance to this disease. These findings can be applied in breeding programs and nutritional management strategies to enhance resistance to soil-borne diseases.</p>