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<p>The discovery of coffee leaf rust (CLR) on Maui and Hawaii Island in late 2020 brought the most devastating coffee disease in the world to the Hawaiian Archipelago. Its rapid spread throughout the state now threatens the survival of commercial coffee production in Hawaii. Coffee is one of the most economically important agricultural crops grown in Hawaii and is produced commercially on six islands across 7,300 acres with a raw value of more than $42.5&#xa0;M USD. <i>Hemileia vastatrix</i>, the obligate parasitic fungus that causes CLR, attacks the leaves of coffee trees, leading to severe defoliation, reduction in photosynthesis, and consequent loss of vigor and yield. High spore load and ease of dispersal make this disease extremely difficult to control and will require early and accurate detection as a critical first step to manage this pathogen when incidence levels are low and symptoms are in the early stages of development. Therefore, a molecular diagnostic real-time PCR protocol using TaqMan probe chemistry to detect targets based on simple sequence repeats (SSR) was developed. The assay provides rapid detection of <i>H. vastatrix</i> using DNA isolated directly from infected coffee leaves and excised spores.</p>

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Real-time PCR assay detection of Hemileia vastatrix, causative agent of coffee leaf rust (CLR)

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • Wade P. Heller,
  • Karma R. Kissinger,
  • Eva Brill,
  • Terry J. Torres-Cruz,
  • M. Catherine Aime,
  • Lisa M. Keith

摘要

The discovery of coffee leaf rust (CLR) on Maui and Hawaii Island in late 2020 brought the most devastating coffee disease in the world to the Hawaiian Archipelago. Its rapid spread throughout the state now threatens the survival of commercial coffee production in Hawaii. Coffee is one of the most economically important agricultural crops grown in Hawaii and is produced commercially on six islands across 7,300 acres with a raw value of more than $42.5 M USD. Hemileia vastatrix, the obligate parasitic fungus that causes CLR, attacks the leaves of coffee trees, leading to severe defoliation, reduction in photosynthesis, and consequent loss of vigor and yield. High spore load and ease of dispersal make this disease extremely difficult to control and will require early and accurate detection as a critical first step to manage this pathogen when incidence levels are low and symptoms are in the early stages of development. Therefore, a molecular diagnostic real-time PCR protocol using TaqMan probe chemistry to detect targets based on simple sequence repeats (SSR) was developed. The assay provides rapid detection of H. vastatrix using DNA isolated directly from infected coffee leaves and excised spores.