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<p>In this study, eight cherry leaf roll virus (CLRV) isolates from Ukrainian elderberry (<i>Sambucus nigra</i>) plants were analyzed to determine their phylogenetic relations with isolates from other countries, the impact of CLRV-infection on yield, quality of berries, activity of ROS scavenging enzymes, and peroxisomes abundance. CLRV-infected plants exhibited symptoms of mosaic and/or leaf rolling. Phylogenetic analysis of a portion of the 3’ UTR showed that the eight isolates (GB MW370509, OQ627297-OQ627303) belonged to clade E. Six isolates had a 98.4–99.0% nucleotide homology with American isolate E804 from <i>S. canadensis</i> (AJ877145). Two isolates Eld18MyrD-Ukr and Eld33MP-Ukr showed the highest identity (93.0%-95.6%) with Polish isolates from <i>Prunus armeniaca</i> (CLRVA2, HM032064 and CLRVA1, HM032063) and with isolate Sn3 from <i>S. nigra</i>, HM165197 (95.6% and 95.3% homology, respectively). The disease caused reduction of berry cluster weight and of the number of clusters per bush relative to healthy plants. The content of anthocyanins, ascorbic acid, and soluble sugars in the berries of CLRV-infected plants were all lower than in healthy plants. Activity of ROS scavengers catalase and peroxidase in berries and abundance of peroxisomes in leaves were also reduced in CLRV-infected plants. Conversely, activity of peroxidase and concentration of flavonoids in leaves was higher. We conclude that the suppression of ROS scavenging system may be an essential component of pathogenesis by CRLV.</p>

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Cherry leaf roll virus on elderberry in Ukraine: phylogeny, impact on yield, berry quality and some links of the immune response

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Alina Dunich,
  • Olga Molodchenkova,
  • Taras Nazarov,
  • Anna Dashchenko,
  • Ivan Mishchenko,
  • Natalia Kozub,
  • Andrei Smertenko,
  • Lidiya Mishchenko

摘要

In this study, eight cherry leaf roll virus (CLRV) isolates from Ukrainian elderberry (Sambucus nigra) plants were analyzed to determine their phylogenetic relations with isolates from other countries, the impact of CLRV-infection on yield, quality of berries, activity of ROS scavenging enzymes, and peroxisomes abundance. CLRV-infected plants exhibited symptoms of mosaic and/or leaf rolling. Phylogenetic analysis of a portion of the 3’ UTR showed that the eight isolates (GB MW370509, OQ627297-OQ627303) belonged to clade E. Six isolates had a 98.4–99.0% nucleotide homology with American isolate E804 from S. canadensis (AJ877145). Two isolates Eld18MyrD-Ukr and Eld33MP-Ukr showed the highest identity (93.0%-95.6%) with Polish isolates from Prunus armeniaca (CLRVA2, HM032064 and CLRVA1, HM032063) and with isolate Sn3 from S. nigra, HM165197 (95.6% and 95.3% homology, respectively). The disease caused reduction of berry cluster weight and of the number of clusters per bush relative to healthy plants. The content of anthocyanins, ascorbic acid, and soluble sugars in the berries of CLRV-infected plants were all lower than in healthy plants. Activity of ROS scavengers catalase and peroxidase in berries and abundance of peroxisomes in leaves were also reduced in CLRV-infected plants. Conversely, activity of peroxidase and concentration of flavonoids in leaves was higher. We conclude that the suppression of ROS scavenging system may be an essential component of pathogenesis by CRLV.