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<p>Peaches hold great importance as a valuable stone fruit crop, yet the complete viral and viroid populations affecting peach varieties in India remain largely unexplored. To address this gap, transcriptome analyses of four rootstocks (Nemaguard, B6, Meripendent and 13–6) and a scion variety (Cresthaven) of peach was performed. Our study identified near- complete genomic sequences of nine viruses, including two ilarviruses: prune dwarf virus (PDV) and prunus necrotic ringspot virus (PNRSV); two luteoviruses: nectarine stem pitting-associated virus (NSPaV) and peach-associated luteovirus (PaLV); one maculavirus: grapevine red globe virus (GRGV); three marafiviruses: grapevine asteroid mosaic-associated virus (GAMaV), peach virus D (PeVD), and citrus sudden death-associated virus (CSDaV); and a novel marafivirus, peach associated marafivirus (PaMV). The accumulation of viral reads varied among these pathogens, with PDV exhibiting the highest proportion (23.54%), followed by PNRSV (9.68%), PaLV (0.25%), and NSPaV (0.20%). The remaining viruses, including GRGV, CSDaV, PaMV, PeVD, and GAMaV, were present at much lower levels (0.03–0.07%). While PNRSV, PDV, NSPaV, PeVD, and PaLV have been previously reported in peach from other countries, the detection of GRGV, CSDaV, and GAMaV suggests peach as a novel host for these viruses. Additionally, PaMV, a marafivirus, represents a novel virus identified in peach. The presence of seven viruses (PDV, PNRSV, GRGV, GAMaV, PeVD, CSDaV, and PaMV) was confirmed through RT-PCR amplification and Sanger sequencing. Furthermore, these seven viruses were validated in 23 peach samples, encompassing indigenous and exotic collections, local varieties, and rootstock collected from research stations and orchards. This study provides valuable information for certification and clean plant programs in India, facilitating the supply of disease-free plants to farmers. The insights gained can be utilized to develop effective virus detection and management strategies, ultimately reducing virus spread and enhancing peach production in India.</p>

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Peach viromics unveils it to be new host of grapevine red globe virus, citrus sudden death-associated virus, grapevine asteroid mosaic-associated virus and a novel marafivirus

  • 期刊论文

  • 发表日期：

    2025年7月15日

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  • Damini Diksha,
  • Malyaj R. Prajapati,
  • Pooja Thapa,
  • Susheel Kumar Sharma,
  • Sajad U. Nabi,
  • Nitika Gupta,
  • Virendra Kumar Baranwal

摘要

Peaches hold great importance as a valuable stone fruit crop, yet the complete viral and viroid populations affecting peach varieties in India remain largely unexplored. To address this gap, transcriptome analyses of four rootstocks (Nemaguard, B6, Meripendent and 13–6) and a scion variety (Cresthaven) of peach was performed. Our study identified near- complete genomic sequences of nine viruses, including two ilarviruses: prune dwarf virus (PDV) and prunus necrotic ringspot virus (PNRSV); two luteoviruses: nectarine stem pitting-associated virus (NSPaV) and peach-associated luteovirus (PaLV); one maculavirus: grapevine red globe virus (GRGV); three marafiviruses: grapevine asteroid mosaic-associated virus (GAMaV), peach virus D (PeVD), and citrus sudden death-associated virus (CSDaV); and a novel marafivirus, peach associated marafivirus (PaMV). The accumulation of viral reads varied among these pathogens, with PDV exhibiting the highest proportion (23.54%), followed by PNRSV (9.68%), PaLV (0.25%), and NSPaV (0.20%). The remaining viruses, including GRGV, CSDaV, PaMV, PeVD, and GAMaV, were present at much lower levels (0.03–0.07%). While PNRSV, PDV, NSPaV, PeVD, and PaLV have been previously reported in peach from other countries, the detection of GRGV, CSDaV, and GAMaV suggests peach as a novel host for these viruses. Additionally, PaMV, a marafivirus, represents a novel virus identified in peach. The presence of seven viruses (PDV, PNRSV, GRGV, GAMaV, PeVD, CSDaV, and PaMV) was confirmed through RT-PCR amplification and Sanger sequencing. Furthermore, these seven viruses were validated in 23 peach samples, encompassing indigenous and exotic collections, local varieties, and rootstock collected from research stations and orchards. This study provides valuable information for certification and clean plant programs in India, facilitating the supply of disease-free plants to farmers. The insights gained can be utilized to develop effective virus detection and management strategies, ultimately reducing virus spread and enhancing peach production in India.