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<p><i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) is a major pathogen in cauliflower, causing yield losses up to 50%. Studying <i>Xcc</i> colonization and infection dynamics is crucial for developing effective disease management strategies. This study aimed to transform <i>Xcc</i> with the green fluorescent protein (GFP)-carrying vector pCAMBIA1302 to visualize black rot causing <i>Xcc</i> colonization in susceptible and resistant cauliflower genotypes under various treatments. <i>Xcc</i> was transformed with pCAMBIA1302 via electroporation, confirmed by confocal laser scanning microscopy (CLSM), GFP gene amplification and <i>hrp</i>-specific primer verification. Cauliflower plants were subjected to five treatments with four replications. Inoculated leaf tissues were examined at 0, 6, 24, 48, 72, and 96&#xa0;h after pathogen inoculation (hpi) using CLSM to monitor GFP-labeled <i>Xcc</i> colonization. GFP-labeled <i>Xcc</i> colonies exhibited strong green fluorescence, confirming successful transformation. In susceptible genotype, <i>Xcc</i> rapidly colonized and reached high populations by 96 hpi, while in resistant genotype, colonization was slower and less extensive, especially under combined treatment with <i>Pseudomonas fluorescens</i> CFLB-27 and acibenzolar-S-methyl (ASM) at 350&#xa0;µg/mL. The highest bacterial populations were observed in <i>Xcc</i> infected control plants. Besides, lowest population was recorded combined treatment of <i>Pseudomonas fluorescens</i> CFLB-27 and ASM at 350 µg/mL, indicating a synergistic effect of biotic and abiotic elicitors in restricting <i>Xcc</i> growth. These results revealed critical insights into pathogen dynamics and treatment efficacy, offering new strategies for improving disease management in cauliflower.</p>

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Temporal dynamics of GFP- labeled Xanthomonas campestris pv. campestris in cauliflower: contrasting responses in resistant and susceptible genotypes to elicitor application

  • 期刊论文

  • 发表日期：

    2025年6月25日

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  • Neelam Geat,
  • Dinesh Singh,
  • Devendra Singh,
  • Rajender Jatoth,
  • Pedapudi Lokesh Babu,
  • G.S. Ramyashree Devi

摘要

Xanthomonas campestris pv. campestris (Xcc) is a major pathogen in cauliflower, causing yield losses up to 50%. Studying Xcc colonization and infection dynamics is crucial for developing effective disease management strategies. This study aimed to transform Xcc with the green fluorescent protein (GFP)-carrying vector pCAMBIA1302 to visualize black rot causing Xcc colonization in susceptible and resistant cauliflower genotypes under various treatments. Xcc was transformed with pCAMBIA1302 via electroporation, confirmed by confocal laser scanning microscopy (CLSM), GFP gene amplification and hrp-specific primer verification. Cauliflower plants were subjected to five treatments with four replications. Inoculated leaf tissues were examined at 0, 6, 24, 48, 72, and 96 h after pathogen inoculation (hpi) using CLSM to monitor GFP-labeled Xcc colonization. GFP-labeled Xcc colonies exhibited strong green fluorescence, confirming successful transformation. In susceptible genotype, Xcc rapidly colonized and reached high populations by 96 hpi, while in resistant genotype, colonization was slower and less extensive, especially under combined treatment with Pseudomonas fluorescens CFLB-27 and acibenzolar-S-methyl (ASM) at 350 µg/mL. The highest bacterial populations were observed in Xcc infected control plants. Besides, lowest population was recorded combined treatment of Pseudomonas fluorescens CFLB-27 and ASM at 350 µg/mL, indicating a synergistic effect of biotic and abiotic elicitors in restricting Xcc growth. These results revealed critical insights into pathogen dynamics and treatment efficacy, offering new strategies for improving disease management in cauliflower.