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<p>Apple blotch, caused by the fungus <i>Diplocarpon coronariae</i>, is a severe disease impacting apple production worldwide, leading to significant yield losses. Current management relies heavily on frequent fungicide applications, which have environmental drawbacks and promote resistance in pathogens. This study aimed to develop apple varieties resistant to apple blotch using a genetic engineering approach, specifically using host-induced gene silencing (HIGS). The HSP90 gene, essential for fungal virulence, was targeted in ‘Red Chief’ apple cultivar, known for its susceptibility to the disease. An RNA interference (RNAi) construct targeting HSP90 was prepared and introduced into apple leaves using Agrobacterium-mediated transformation. Regenerated putative shoots were screened, and transgenic lines confirmed via PCR and RT-qPCR for the integration and expression of the RNAi construct. Detached leaf assays showed reduced disease symptoms in transgenic lines compared to wild-type controls. Microscopic analysis revealed limited fungal colonization in RNAi transgenic lines. This study demonstrates the potential of HIGS targeting HSP90 for developing apple varieties with enhanced resistance to apple blotch, offering a sustainable alternative to chemical control methods.</p>

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Host delivered RNA interference in apple cv. Red Chief for mediating resistance against Diplocarpon coronariae

  • 期刊论文

  • 发表日期：

    2025年7月7日

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  • Abhishek Kumar,
  • Neha Kumari,
  • Arjun Chauhan,
  • Manju Modgil

摘要

Apple blotch, caused by the fungus Diplocarpon coronariae, is a severe disease impacting apple production worldwide, leading to significant yield losses. Current management relies heavily on frequent fungicide applications, which have environmental drawbacks and promote resistance in pathogens. This study aimed to develop apple varieties resistant to apple blotch using a genetic engineering approach, specifically using host-induced gene silencing (HIGS). The HSP90 gene, essential for fungal virulence, was targeted in ‘Red Chief’ apple cultivar, known for its susceptibility to the disease. An RNA interference (RNAi) construct targeting HSP90 was prepared and introduced into apple leaves using Agrobacterium-mediated transformation. Regenerated putative shoots were screened, and transgenic lines confirmed via PCR and RT-qPCR for the integration and expression of the RNAi construct. Detached leaf assays showed reduced disease symptoms in transgenic lines compared to wild-type controls. Microscopic analysis revealed limited fungal colonization in RNAi transgenic lines. This study demonstrates the potential of HIGS targeting HSP90 for developing apple varieties with enhanced resistance to apple blotch, offering a sustainable alternative to chemical control methods.