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<p><i>Botrytis cinerea</i> is responsible for gray mold disease in several economically important crops, including strawberry, tomato, grape, and cucumber, establishing it as a globally significant pathogen. Resistance to succinate dehydrogenase inhibitor (SDHI) fungicides has been linked to single-point mutations in the <i>sdhB</i> gene, with nine mutations reported to date. In this study, we developed dCAPS markers that successfully differentiate seven key mutations (P225F/T/L, N230I, and H272R/Y/L) in SdhB by analyzing restriction enzyme digestion patterns. Field validation with <i>B. cinerea</i> isolates from infected strawberries in Shanghai demonstrated high specificity and reliability, with a 100% accuracy compared to sequencing-based validation. This PCR-based method will assist in the continuous monitoring and effective management of SDHI resistance in <i>B. cinerea</i>. Future applications could expand this approach to other fungal pathogens and integrate it with real-time diagnostics for enhanced disease control in both pre- and post-harvest settings.</p>

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Development of dCAPS markers for sdhB mutants in fungicide-resistant Botrytis cinerea isolates

  • 期刊论文

  • 发表日期：

    2025年8月18日

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  • Rong Zeng,
  • Ping Gao,
  • Lihui Xu,
  • Ya’nan Yuan,
  • Yufei Han,
  • Jiaxi Tian,
  • Yuhong Wei,
  • Shigang Gao,
  • Zhiwei Song,
  • Pu Zhu,
  • Fuming Dai

摘要

Botrytis cinerea is responsible for gray mold disease in several economically important crops, including strawberry, tomato, grape, and cucumber, establishing it as a globally significant pathogen. Resistance to succinate dehydrogenase inhibitor (SDHI) fungicides has been linked to single-point mutations in the sdhB gene, with nine mutations reported to date. In this study, we developed dCAPS markers that successfully differentiate seven key mutations (P225F/T/L, N230I, and H272R/Y/L) in SdhB by analyzing restriction enzyme digestion patterns. Field validation with B. cinerea isolates from infected strawberries in Shanghai demonstrated high specificity and reliability, with a 100% accuracy compared to sequencing-based validation. This PCR-based method will assist in the continuous monitoring and effective management of SDHI resistance in B. cinerea. Future applications could expand this approach to other fungal pathogens and integrate it with real-time diagnostics for enhanced disease control in both pre- and post-harvest settings.