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<p>The research aims to characterize ethnomedicinal mushroom <i>Lentinus squarrosulus</i> isolates through morphological and molecular approaches. The nutritional requirements and cultural preferences required for the growth of <i>L. squarrosulus</i> were studied to maximize the production and its potential values. Morphological features of <i>Lentinus</i> sp. isolates were observed, molecular identification was performed using ITS primer, and further DNA sequencing confirmed to identify at the species level as <i>Lentinus squarrosulus</i>. Culturing the isolates was carried out on potato dextrose agar (PDA) for assessing cultural traits, colony diameter, and mycelial dry weight, in addition to assessing the impact of various growth factors like carbon and nitrogen sources, pH, and temperature on mycelial growth. Five isolates of <i>L. squarrosulus</i> were studied in that LS3 demonstrated the best growth parameters, with PDA as the most conducive medium amended with 4% sorbitol and 4% mannitol as optimal carbon sources. Furthermore, maintaining pH levels between 6.0 and 7.0 and temperatures ranging from 25&#xa0;°C to 30&#xa0;°C were maintained for mycelial growth. The results highlight the crucial significance of comprehending the growth requirements of this mushroom species to effectively maximize its cultivation potential. Further exploration of these findings may unveil behavioral aspects of <i>L. squarrosulus</i>, including alterations in enzyme profiles and phenolic content, potentially fostering applications in biotechnology.</p>

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Unveiling the ethnomedicinal mystery of Lentinus squarrosulus Mont.: a multi-dimensional exploration of morphological, molecular profiling and optimization of cultural and nutritional conditions

  • 期刊论文

  • 发表日期：

    2025年6月18日

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  • K. Ganesh Saravanan,
  • G. Thiribhuvanamala,
  • T. Praveen,
  • K. Angappan,
  • N. Revathy,
  • R. Anandham

摘要

The research aims to characterize ethnomedicinal mushroom Lentinus squarrosulus isolates through morphological and molecular approaches. The nutritional requirements and cultural preferences required for the growth of L. squarrosulus were studied to maximize the production and its potential values. Morphological features of Lentinus sp. isolates were observed, molecular identification was performed using ITS primer, and further DNA sequencing confirmed to identify at the species level as Lentinus squarrosulus. Culturing the isolates was carried out on potato dextrose agar (PDA) for assessing cultural traits, colony diameter, and mycelial dry weight, in addition to assessing the impact of various growth factors like carbon and nitrogen sources, pH, and temperature on mycelial growth. Five isolates of L. squarrosulus were studied in that LS3 demonstrated the best growth parameters, with PDA as the most conducive medium amended with 4% sorbitol and 4% mannitol as optimal carbon sources. Furthermore, maintaining pH levels between 6.0 and 7.0 and temperatures ranging from 25 °C to 30 °C were maintained for mycelial growth. The results highlight the crucial significance of comprehending the growth requirements of this mushroom species to effectively maximize its cultivation potential. Further exploration of these findings may unveil behavioral aspects of L. squarrosulus, including alterations in enzyme profiles and phenolic content, potentially fostering applications in biotechnology.