Scholarlink

<p><i>Fusarium</i> root and stem rot, caused by <i>Fusarium oxysporum</i>, significantly reduced cucumber production. The use of chemical fungicides as control agents provides a temporary remedy; however, their detrimental long-term effects on the environment and human health are considerable, and they also foster fungal resistance. Consequently, sustainable alternatives must be devised. This study isolated <i>Bacillus licheniformis</i> AD70 from soil, identified it by 16&#xa0;S rRNA gene sequencing, and utilized it to generate bacteriogenic selenium nanoparticles (SeNPs) as an alternative fungicide for controlling <i>Fusarium</i> root rot and stem rot in cucumber. Bacteriogenic SeNPs had a spherical shape, with 32&#xa0;nm in diameter, and possessed a negative charge of -25.5 mV. The SeNPs showed significant antioxidant activity, neutralizing 91% of DPPH and suppressing the proliferation of many cucumber pathogens, including <i>F. oxysporum</i>, in a concentration-dependent manner. In the greenhouse experiment, the foliar application of SeNPs (75&#xa0;mg/L) significantly (<i>P</i> &lt; 0.05) diminished 96% of the incidence of <i>Fusarium</i> root rot and stem rot by upregulating (<i>P</i> &lt; 0.05) the expression of key genes [Lipoxygenase (LOX), pathogenesis-related protein 1 (PR1a), and phenylalanine ammonia-lyase (PAL)], which activate systemic acquired resistance and growth hormones in comparison to infected plants. As a result, the antioxidant enzymes catalase (CAT), peroxidase (POD), and polyphenol oxidase (PPO) were reduced by 700%, 193%, and 86%, respectively, in comparison to infected cucumbers; concurrently, the chlorophyll and carotenoid content significantly (<i>P</i> &lt; 0.05) increased by 218% and 67%, respectively, in SeNPs-treated cucumber plants relative to infected plants. The foliar application of SeNPs (75&#xa0;mg/L) significantly improved cucumber fruit quality and quantity, resulting in a 210% increase in the number of fruits and a 12% increase in fruit weight, hence enhancing the net yield by 120% compared to infested cucumbers. Bacteriogenic SeNPs produced by <i>B. licheniformis</i> AD70 are proposed as an alternative biocontrol agent against <i>F. oxysporum</i> and potentially other phytopathogenic fungi in cucumber under greenhouse conditions, likely via a combination of antifungal activity and induced systemic resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The use of selenium nanoparticles from Bacillus licheniformis AD70 to enhance cucumber defense genes against Fusarium root and stem rot disease and increase antifungal activity, antioxidant stability, and fruit yield

  • 期刊论文

  • 发表日期：

    2025年7月4日

查看全文
  • Latifa Al Husnain,
  • Hayfa Habes Almutairi,
  • Fatma Mohamed Ameen Khalil,
  • Soha A. Alamoudi,
  • Mohammed O. Alshaharni,
  • Atef Fathy Ahmed,
  • Belal A. Omar,
  • Hend A. Elakkad,
  • Sally Attia,
  • Maha M. Nader

摘要

Fusarium root and stem rot, caused by Fusarium oxysporum, significantly reduced cucumber production. The use of chemical fungicides as control agents provides a temporary remedy; however, their detrimental long-term effects on the environment and human health are considerable, and they also foster fungal resistance. Consequently, sustainable alternatives must be devised. This study isolated Bacillus licheniformis AD70 from soil, identified it by 16 S rRNA gene sequencing, and utilized it to generate bacteriogenic selenium nanoparticles (SeNPs) as an alternative fungicide for controlling Fusarium root rot and stem rot in cucumber. Bacteriogenic SeNPs had a spherical shape, with 32 nm in diameter, and possessed a negative charge of -25.5 mV. The SeNPs showed significant antioxidant activity, neutralizing 91% of DPPH and suppressing the proliferation of many cucumber pathogens, including F. oxysporum, in a concentration-dependent manner. In the greenhouse experiment, the foliar application of SeNPs (75 mg/L) significantly (P < 0.05) diminished 96% of the incidence of Fusarium root rot and stem rot by upregulating (P < 0.05) the expression of key genes [Lipoxygenase (LOX), pathogenesis-related protein 1 (PR1a), and phenylalanine ammonia-lyase (PAL)], which activate systemic acquired resistance and growth hormones in comparison to infected plants. As a result, the antioxidant enzymes catalase (CAT), peroxidase (POD), and polyphenol oxidase (PPO) were reduced by 700%, 193%, and 86%, respectively, in comparison to infected cucumbers; concurrently, the chlorophyll and carotenoid content significantly (P < 0.05) increased by 218% and 67%, respectively, in SeNPs-treated cucumber plants relative to infected plants. The foliar application of SeNPs (75 mg/L) significantly improved cucumber fruit quality and quantity, resulting in a 210% increase in the number of fruits and a 12% increase in fruit weight, hence enhancing the net yield by 120% compared to infested cucumbers. Bacteriogenic SeNPs produced by B. licheniformis AD70 are proposed as an alternative biocontrol agent against F. oxysporum and potentially other phytopathogenic fungi in cucumber under greenhouse conditions, likely via a combination of antifungal activity and induced systemic resistance.