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<p>To develop a vaccine against cucumber mosaic virus (CMV), a mutant strain (CM14-1) was obtained during the passage of a wild-type strain. When CM14-1 infects cucumbers, it produces mild symptoms and achieves systemic infection slowly. While passaging the CM14-1, a mutant strain CM14-2 was obtained, which maintained its attenuated symptoms but had restored speed of systemic infection. In this study, we aimed to elucidate the mechanism attenuating the virulence and/or rate of systemic infection of these isolates. CM14-1 had a deletion mutation at the 3′ end of the 2b gene, and the predicted amino acid sequence was approximately 50% longer than that of the wild type due to a frameshift. Meanwhile, CM14-2 had a nonsense mutation downstream of the deletion mutation, and the predicted amino acid sequence was slightly shorter than that of the wild-type strain. Analysis of RNA silencing suppressor (RSS) ability revealed that 2b in CM14-1 had weak RSS ability, while the RSS ability of 2b in CM14-2 was as strong as that of the wild-type strain. CM14-2 showed cross-protection against wild-type CMV. These results suggest that the C-terminal region of the 2b protein is involved in RSS function, and that CM14-2 may be useful as a novel vaccine for the control of viral diseases.</p>

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New attenuated strains of cucumber mosaic virus possess mutations in the C-terminal region of the 2b protein

  • 期刊论文

  • 发表日期：

    2025年4月29日

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  • Risa Shiono,
  • Hiromitsu Seki,
  • Ryo Teranishi,
  • Takaaki Mashiko,
  • Yutaro Neriya,
  • Hisashi Nishigawa,
  • Shigemitsu Kimura,
  • Boseong Yang,
  • Tomohide Natsuaki

摘要

To develop a vaccine against cucumber mosaic virus (CMV), a mutant strain (CM14-1) was obtained during the passage of a wild-type strain. When CM14-1 infects cucumbers, it produces mild symptoms and achieves systemic infection slowly. While passaging the CM14-1, a mutant strain CM14-2 was obtained, which maintained its attenuated symptoms but had restored speed of systemic infection. In this study, we aimed to elucidate the mechanism attenuating the virulence and/or rate of systemic infection of these isolates. CM14-1 had a deletion mutation at the 3′ end of the 2b gene, and the predicted amino acid sequence was approximately 50% longer than that of the wild type due to a frameshift. Meanwhile, CM14-2 had a nonsense mutation downstream of the deletion mutation, and the predicted amino acid sequence was slightly shorter than that of the wild-type strain. Analysis of RNA silencing suppressor (RSS) ability revealed that 2b in CM14-1 had weak RSS ability, while the RSS ability of 2b in CM14-2 was as strong as that of the wild-type strain. CM14-2 showed cross-protection against wild-type CMV. These results suggest that the C-terminal region of the 2b protein is involved in RSS function, and that CM14-2 may be useful as a novel vaccine for the control of viral diseases.