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<p>Youcai mosaic virus (YoMV) is a member of the genus <i>Tobamovirus</i>, and mainly infects Brassicaceae plants. In 2017, we collected <i>Chelidonium majus</i> var. <i>asiaticum</i> showing chlorosis and vein banding symptoms in Chuncheon, Korea. RT-PCR results using total RNA of <i>C. majus</i> var. <i>asiaticum</i> showed the presence of a tobamovirus. The RT-PCR product was shown to be YoMV by sequencing (named YoMV-Chm). To fulfill Koch’s postulates, YoMV-Chm was obtained by single local lesion isolation and mechanically inoculated to two <i>C. majus</i> var. <i>asiaticum</i>. At 14&#xa0;days post-inoculation, YoMV-Chm did not induce any symptoms, but systemic infection was confirmed by RT-PCR in <i>C. majus</i> var. asiaticum. In molecular characterization, the complete genome of YoMV-Chm was sequenced and analyzed (GenBank Accession No. LC390003). The genome structure of YoMV-Chm was like typical YoMV isolates. In sequence analysis with other YoMV isolates, YoMV-Chm is most closely related to YoMV isolates from Brassicaceae plants. In biological testing, YoMV-Chm was inoculated to ten indicator species, including Brassicaceae crops, and systemically infected all Brassicaceae crops tested. To investigate the transmission of the virus, seed transmission rates of YoMV-Chm were tested using <i>C. majus</i> var. <i>asiaticum</i>. YoMV-Chm was detected on the seeds; however, YoMV was not transmitted by seeds like other <i>Tobamovirus</i> species. Overall, this is first report of YoMV isolated from <i>C. majus</i> var. <i>asiaticum</i> and its characteristics.</p>

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Characterization of youcai mosaic virus isolated from Chelidonium majus var. asiaticum in Korea

  • 期刊论文

  • 发表日期：

    2025年2月18日

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  • Seong-An Choi,
  • Tae-Seon Park,
  • Ji-Soo Park,
  • Dong-Joo Min,
  • Bong-Chun Lee,
  • Sang-Min Kim,
  • Won-Kyong Cho,
  • Jin-Sung Hong

摘要

Youcai mosaic virus (YoMV) is a member of the genus Tobamovirus, and mainly infects Brassicaceae plants. In 2017, we collected Chelidonium majus var. asiaticum showing chlorosis and vein banding symptoms in Chuncheon, Korea. RT-PCR results using total RNA of C. majus var. asiaticum showed the presence of a tobamovirus. The RT-PCR product was shown to be YoMV by sequencing (named YoMV-Chm). To fulfill Koch’s postulates, YoMV-Chm was obtained by single local lesion isolation and mechanically inoculated to two C. majus var. asiaticum. At 14 days post-inoculation, YoMV-Chm did not induce any symptoms, but systemic infection was confirmed by RT-PCR in C. majus var. asiaticum. In molecular characterization, the complete genome of YoMV-Chm was sequenced and analyzed (GenBank Accession No. LC390003). The genome structure of YoMV-Chm was like typical YoMV isolates. In sequence analysis with other YoMV isolates, YoMV-Chm is most closely related to YoMV isolates from Brassicaceae plants. In biological testing, YoMV-Chm was inoculated to ten indicator species, including Brassicaceae crops, and systemically infected all Brassicaceae crops tested. To investigate the transmission of the virus, seed transmission rates of YoMV-Chm were tested using C. majus var. asiaticum. YoMV-Chm was detected on the seeds; however, YoMV was not transmitted by seeds like other Tobamovirus species. Overall, this is first report of YoMV isolated from C. majus var. asiaticum and its characteristics.