Scholarlink

<p>Cucumber mosaic virus (CMV; <i>Cucumovirus CMV</i>, family <i>Bromoviridae</i>) is among the top prevalent plant-infecting viruses and it is a ruinous agent in diverse crops. During 2022 and 2023, thirty-five rapeseed leaf samples exhibiting mosaic, vein yellows, and deformation were collected from Basrah Province, Iraq. Serological assays confirmed CMV infection in 13 out of 35 samples (37.14%). Based on host range tests, Iraqi CMV isolates were grouped into two distinct mild and severe biotypes. Recombination analysis detected a breakpoint within the <i>CP</i> gene of three Iraqi CMV isolates, which cluster into a distinct phylogroup in the phylogenetic tree. This finding suggests the presence of distinctly evolving strains that differ from previously reported CMV isolates. The relative codon deoptimization index values revealed the highest adaptation of CMV to tobacco plants. Furthermore, the similarity index indicated that the rapeseed host exerted greater evolutionary pressure on CMV, suggesting that some hosts may play a more important role in the emergence of new CMV strains. This is the first report of the natural occurrence of a recombinant CMV isolate from Southern-Eurasia, Iraq.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological and molecular characterization of recombinant cucumber mosaic virus (Cucumovirus CMV) isolates from rapeseed in Southern-Eurasia Iraq

  • 期刊论文

  • 发表日期：

    2025年2月24日

查看全文
  • Shirin Farzadfar,
  • Muhannad Al-Waeli,
  • Reza Pourrrahim

摘要

Cucumber mosaic virus (CMV; Cucumovirus CMV, family Bromoviridae) is among the top prevalent plant-infecting viruses and it is a ruinous agent in diverse crops. During 2022 and 2023, thirty-five rapeseed leaf samples exhibiting mosaic, vein yellows, and deformation were collected from Basrah Province, Iraq. Serological assays confirmed CMV infection in 13 out of 35 samples (37.14%). Based on host range tests, Iraqi CMV isolates were grouped into two distinct mild and severe biotypes. Recombination analysis detected a breakpoint within the CP gene of three Iraqi CMV isolates, which cluster into a distinct phylogroup in the phylogenetic tree. This finding suggests the presence of distinctly evolving strains that differ from previously reported CMV isolates. The relative codon deoptimization index values revealed the highest adaptation of CMV to tobacco plants. Furthermore, the similarity index indicated that the rapeseed host exerted greater evolutionary pressure on CMV, suggesting that some hosts may play a more important role in the emergence of new CMV strains. This is the first report of the natural occurrence of a recombinant CMV isolate from Southern-Eurasia, Iraq.