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<p>Apple rubbery wood virus 1 (ARWV-1, genus <i>Rubodvirus</i>, family <i>Phenuiviridae</i>) is a causal agent of rubbery wood disease, and is also associated with flat limb disease in apple. ARWV-1 has been detected in apple trees in China, however, its complete genome sequences remain unreported. Here, we report the complete nucleotide sequences of three isolates of ARWV-1 (FJ, XH, and XF) collected from apple trees in Xingcheng, Liaoning Province, China. Their genomes contain three RNA segments: L, M, and S. Sequence alignment showed that the genomic sequences of FJ, XH, and XF have 94.3–99.6%, 93.6–99.8%, and 84.5–99.8% nucleotide identity, respectively, with the five previously reported ARWV-1 isolates. Phylogenetic analyses based on the nucleotide sequences of the L, M, and S segments indicated that all ARWV-1 full genome isolates could be divided into three clades. In phylogenetic trees for each genome segment, isolates FJ, XH, and XF formed a clade with isolate BR-Mishima from Brazil. This is the first report of complete genome sequences of ARWV-1 from apple trees in China. Our results will provide important reference information for the prevention and control of this virus.</p>

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Molecular characteristics of three complete genome sequences of apple rubbery wood virus 1 isolates from apple trees in China

  • 期刊论文

  • 发表日期：

    2025年1月20日

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  • Cheng-long Liu,
  • Zun-Ping Zhang,
  • Xu-Dong Fan,
  • Fang Ren,
  • Guo-Jun Hu,
  • Ya-Feng Dong

摘要

Apple rubbery wood virus 1 (ARWV-1, genus Rubodvirus, family Phenuiviridae) is a causal agent of rubbery wood disease, and is also associated with flat limb disease in apple. ARWV-1 has been detected in apple trees in China, however, its complete genome sequences remain unreported. Here, we report the complete nucleotide sequences of three isolates of ARWV-1 (FJ, XH, and XF) collected from apple trees in Xingcheng, Liaoning Province, China. Their genomes contain three RNA segments: L, M, and S. Sequence alignment showed that the genomic sequences of FJ, XH, and XF have 94.3–99.6%, 93.6–99.8%, and 84.5–99.8% nucleotide identity, respectively, with the five previously reported ARWV-1 isolates. Phylogenetic analyses based on the nucleotide sequences of the L, M, and S segments indicated that all ARWV-1 full genome isolates could be divided into three clades. In phylogenetic trees for each genome segment, isolates FJ, XH, and XF formed a clade with isolate BR-Mishima from Brazil. This is the first report of complete genome sequences of ARWV-1 from apple trees in China. Our results will provide important reference information for the prevention and control of this virus.