Genetic diversity of Erwinia amylovora isolates from fire blight diseased trees in Central and Eastern Georgia
摘要
The genetic diversity of 52 Erwinia amylovora isolates from pome fruit trees with fire blight in Central and Eastern Georgia from the years 2020–2022 was examined using specific PCR and genotyping of CRISPR repeat regions 1 and 2. The analysis identified three distinct genotypes: (A, a, α), matching historical Western European strains; (A, z, α), distinctive for Georgia and differing by a three-spacer deletion in CRR2 (2034–2036); as well as novel genotype (A, ä, α), which was not observed previously. Genotypes (A, a, α) and (A, z, α) were found to coexist geographically in all four regions investigated, whereas genotype (A, ä, α) was reported only in one case in the region of Kvemo Kartli. On separate instances, multiple genotypes were detected even within the same orchard or tree, illustrating the complex genetic landscape of E. amylovora in the country.