<p>Electrospun nanofibers, with their unique physical and mechanical properties, exhibit substantial potential in applications related to real-time motion tracking and health monitoring. This review highlights their use in wearable sensors, motion detection, and health monitoring devices, while addressing the challenges and opportunities for clinical translation and commercialization. These nanofibers provide high sensitivity and flexibility, facilitating the real-time monitoring of parameters such as gait patterns, running posture, and electrocardiogram signals. Their integration into sports training and rehabilitation enhances performance assessment and feedback, thereby improving training efficacy and hastening recovery periods. Furthermore, health monitoring devices utilizing electrospun nanofibers offer comfort and portability, making them effective tools for personal health management and medical diagnosis. The paper also discusses challenges such as large-scale production, reproducibility, and regulatory issues, proposing potential solutions. Future directions include developing new materials, leveraging advanced manufacturing technologies, and exploring new application areas. With ongoing technological advancements and market demand, electrospun nanofibers are poised to significantly impact motion monitoring devices, enhancing their intelligence, convenience, and efficiency.</p>