<p>Healthcare personnel in infectious wards are exposed to bioaerosols, necessitating the use of respirators and personal protective equipment (PPE) to prevent pathogen contact. It is critical to evaluate and enhance the efficiency of portable, commercially available Powered Air Purifying Respirator (PAPR) devices to mitigate the risk of airborne microbial infiltering into the human respiratory system. In this study, a portable, cost-effective, and lightweight ultraviolet (UV) disinfection system was designed and developed. The system actively draws in air, effectively inactivates microorganisms, and releases purified air. The modular HEPA-UVC conjugated PAPR device developed by us can be attached to any existing respirator unit. The device was tested for filtering airborne microbes, mainly bacteria and fungi. Further, the efficiency of the device was assessed by calculating the percentage of airborne bacteria cells and fungal spores. The microbial sampling results indicated that UVC treatment along with HEPA filter operated for 15 min could achieve a 92.63% reduction of bacteria and an 80.43% reduction of fungi as compared to the HEPA filter alone. The time of exposure and flow rate were the dominant parameters affecting the number of bioaerosols in the disinfection performance of a UVC lamp. Thus, HEPA air filtration combined with UVC provided an efficient reduction of airborne pathogens from indoor air. The deployment of such integrated systems is extremely useful to provide cleaner air in infectious wards. These portable devices are a viable method to improve safeguarding healthcare personnel against pathogen infection and prevent the spread of airborne diseases.</p>