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<p>Nano particles prepared via biological extracts show diverse properties, leading to wide range of applicability in many areas. This study provides the comparative properties of ZnO nanoparticles (ZnO NPs) synthesised using extracts of Honey and Nigella sativa seeds, by the sol-gel route. The XRD revealed a hexagonal wurtzite structure, and the crystallite size for Nigella sativa seed-based ZnO (B) was 32.25 nm, whereas honey-based ZnO (H) was 25.31 nm. The Zn-O bond stretching and other vibrations were confirmed through FTIR spectra. The absorption edge and optical properties were verified by UV-visible spectroscopy and absorption peaks were observed at 350 nm for (ZnO (H)) and 352 nm for (ZnO (B)). Raman spectra exhibited broadened phonon bands for ZnO (H), probably due to phonon confinement, and for ZnO (B), a blue shift was observed due to organic residues in Nigella sativa seeds. SEM-EDX revealed the spherical morphology for both biological extracts-based nanoparticles, and the exclusive presence of Zn and O elements attests to the purity of the samples. The synthesised ZnO nanoparticles were applied for latent fingerprint development using the dusting method. ZnO (H) NPs showed proficient visibility of fingerprints and ridge characteristics on different surfaces. Antimicrobial activity was done through the agar well diffusion method on ZnO NPs for the gram-positive, gram-negative bacterial strains and fungal strains. The ZnO (H) sample showed a maximum inhibition zone for Minimum Inhibition Concentration (MIC) of 0.5% against bacteria and 1% against fungi. ZnO (H) NPs illustrated efficient properties and applications than ZnO (B) NPs.</p>

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Natural extract-based ZnO nanoparticles for forensic and antimicrobial investigations

  • 期刊论文

  • 发表日期：

    2026年6月16日

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  • M. Deepika,
  • G. Tejaswini,
  • V. Mounika,
  • N. Pavan. Kumar,
  • G. Lalitha,
  • T. Sowmyya,
  • Chandrasekhar Maalegoundla

摘要

Nano particles prepared via biological extracts show diverse properties, leading to wide range of applicability in many areas. This study provides the comparative properties of ZnO nanoparticles (ZnO NPs) synthesised using extracts of Honey and Nigella sativa seeds, by the sol-gel route. The XRD revealed a hexagonal wurtzite structure, and the crystallite size for Nigella sativa seed-based ZnO (B) was 32.25 nm, whereas honey-based ZnO (H) was 25.31 nm. The Zn-O bond stretching and other vibrations were confirmed through FTIR spectra. The absorption edge and optical properties were verified by UV-visible spectroscopy and absorption peaks were observed at 350 nm for (ZnO (H)) and 352 nm for (ZnO (B)). Raman spectra exhibited broadened phonon bands for ZnO (H), probably due to phonon confinement, and for ZnO (B), a blue shift was observed due to organic residues in Nigella sativa seeds. SEM-EDX revealed the spherical morphology for both biological extracts-based nanoparticles, and the exclusive presence of Zn and O elements attests to the purity of the samples. The synthesised ZnO nanoparticles were applied for latent fingerprint development using the dusting method. ZnO (H) NPs showed proficient visibility of fingerprints and ridge characteristics on different surfaces. Antimicrobial activity was done through the agar well diffusion method on ZnO NPs for the gram-positive, gram-negative bacterial strains and fungal strains. The ZnO (H) sample showed a maximum inhibition zone for Minimum Inhibition Concentration (MIC) of 0.5% against bacteria and 1% against fungi. ZnO (H) NPs illustrated efficient properties and applications than ZnO (B) NPs.