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<p>Hydroxyapatite is a widely explored bio ceramic, but achieving controlled nanorod morphology remains challenging with conventional synthetic routes. This research focuses on synthesizing hydroxyapatite through a novel method using the biopolymers chitosan and cinnamaldehyde for Schiff base template formation. This template guided the mineralization of phosphate and calcium precursors, thus producing well defined hydroxyapatite nanorods with an aspect ratio from 2.5 to 4 as confirmed by SEM analysis. Structural and chemical analysis verified that the synthesized HA is comparable to commercial HA (HA_C). The cytotoxicity of these nanorods using the MTT and live/dead assays on murine fibroblast cells and mesenchymal stem cells showed &gt; 70% viability over 7 days, indicating non toxicity and enhanced proliferation. The osteogenic potential of the hydroxyapatite was confirmed using alkaline phosphatase and ARS staining, where initiation and completion of mineralization was confirmed. Hence, this work introduces a distinctive, template driven approach for synthesizing biologically active HA nanorods, offering a significant improvement over existing commercial hydroxyapatite.</p>

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Chitosan-derived Schiff base and its application as a template for the synthesis of hydroxyapatite nanorods

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Anupama Devi Vijayan Kumary,
  • Amit Kumar Jaiswal

摘要

Hydroxyapatite is a widely explored bio ceramic, but achieving controlled nanorod morphology remains challenging with conventional synthetic routes. This research focuses on synthesizing hydroxyapatite through a novel method using the biopolymers chitosan and cinnamaldehyde for Schiff base template formation. This template guided the mineralization of phosphate and calcium precursors, thus producing well defined hydroxyapatite nanorods with an aspect ratio from 2.5 to 4 as confirmed by SEM analysis. Structural and chemical analysis verified that the synthesized HA is comparable to commercial HA (HA_C). The cytotoxicity of these nanorods using the MTT and live/dead assays on murine fibroblast cells and mesenchymal stem cells showed > 70% viability over 7 days, indicating non toxicity and enhanced proliferation. The osteogenic potential of the hydroxyapatite was confirmed using alkaline phosphatase and ARS staining, where initiation and completion of mineralization was confirmed. Hence, this work introduces a distinctive, template driven approach for synthesizing biologically active HA nanorods, offering a significant improvement over existing commercial hydroxyapatite.